Related Experiment Video

Updated: Jun 18, 2026

Stimulation of Notch Signaling in Mouse Osteoclast Precursors
08:01

Stimulation of Notch Signaling in Mouse Osteoclast Precursors

Published on: February 28, 2017

Heterogeneity of Jagged1 expression in human and mouse intestinal tumors: implications for targeting Notch signaling

S Guilmeau1, M Flandez, J M Mariadason

  • 1Oncology Department, Albert Einstein Cancer Center, Montefiore Medical Center, Bronx, NY, USA. sandra.guilmeau@inserm.fr

Oncogene
|November 26, 2009
PubMed

Insights

Targeting Jagged1, a Notch pathway ligand, shows promise for colon cancer treatment. This approach may reduce tumor growth while minimizing side effects associated with broader Notch pathway inhibition.

Area of Science:

  • Oncology
  • Cell Signaling
  • Gastrointestinal Cancer

Background:

  • Notch signaling inhibition is a known strategy for colon tumorigenesis.
  • Targeting specific pathway components may offer improved therapeutic strategies.
  • Jagged1, a Notch ligand, plays a role in this pathway.

Purpose of the Study:

  • To investigate the role and expression of Jagged1 in colon cancer.
  • To determine if Jagged1 can be a viable therapeutic target.
  • To assess the potential of Jagged1 targeting to mitigate Notch signaling toxicity.

Main Methods:

  • Analysis of Jagged1 expression in human and mouse intestinal tissues and tumors.
  • Correlation of Jagged1 expression with Wnt signaling and Notch pathway activation.
  • In vitro studies using HT29Cl16E colonic carcinoma cell line to examine goblet cell differentiation and Notch activation.

Main Results:

  • Jagged1 expression is restricted in normal intestine but elevated in human colon tumors and mouse intestinal tumors.
  • Elevated Jagged1 correlates with increased Notch signaling in mouse models.
  • Loss of Jagged1-mediated Notch activation is crucial for goblet cell differentiation in a colon cancer cell line.

Conclusions:

  • Jagged1 is upregulated in a subset of colon tumors, suggesting it as a potential therapeutic target.
  • Targeting Jagged1 could effectively downregulate Notch signaling in these tumors.
  • This targeted approach may offer an alternative to broad Notch inhibition, potentially reducing gastrointestinal toxicity.

More Related Videos

Integration of Bioinformatics Approaches and Experimental Validations to Understand the Role of Notch Signaling in Ovarian Cancer09:08

Integration of Bioinformatics Approaches and Experimental Validations to Understand the Role of Notch Signaling in Ovarian Cancer

Published on: January 12, 2020

Determining Bile Duct Density in the Mouse Liver07:35

Determining Bile Duct Density in the Mouse Liver

Published on: April 30, 2019

Related Concept Videos

Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Notch Signaling Pathway03:14

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
Notch Signaling Pathway03:14

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
Hedgehog Signaling Pathway02:33

Hedgehog Signaling Pathway

The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
Mouse Models of Cancer Study02:43

Mouse Models of Cancer Study

Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...
Mouse Models of Cancer Study02:43

Mouse Models of Cancer Study

Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...