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Related Experiment Video

Updated: Jun 18, 2026

Assessment of Zebrafish Lens Nucleus Localization and Sutural Integrity
07:16

Assessment of Zebrafish Lens Nucleus Localization and Sutural Integrity

Published on: May 6, 2019

The zebrafish lens proteome during development and aging.

Teri M S Greiling1, Scott A Houck, John I Clark

  • 1Department of Biological Structure, University of Washington, Seattle, WA 98195-7420, USA.

Molecular Vision
|November 26, 2009
PubMed
Summary

Zebrafish lens crystallin expression changes with age, with alpha- and gamma-crystallins increasing and novel crystallins identified. This age-related protein shift is crucial for maintaining optical function.

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Area of Science:

  • Ophthalmology
  • Developmental Biology
  • Proteomics

Background:

  • Lens protein expression is vital for optical clarity.
  • Age-related changes in mammalian lens crystallins are known, but poorly understood in zebrafish.

Purpose of the Study:

  • To systematically analyze age-related changes in zebrafish lens protein expression.
  • To understand the role of crystallins in zebrafish lens development and aging.

Main Methods:

  • Size exclusion chromatography (SEC) and linear trap quadrupole Fourier transform tandem mass spectrometry (LTQ-FT LC-MS/MS) proteomics were used.
  • Protein content was analyzed in zebrafish lenses across different life stages (larval, juvenile, adult).

Main Results:

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Lens Transplantation in Zebrafish and its Application in the Analysis of Eye Mutants
10:39

Lens Transplantation in Zebrafish and its Application in the Analysis of Eye Mutants

Published on: June 1, 2009

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Last Updated: Jun 18, 2026

Assessment of Zebrafish Lens Nucleus Localization and Sutural Integrity
07:16

Assessment of Zebrafish Lens Nucleus Localization and Sutural Integrity

Published on: May 6, 2019

Lens Transplantation in Zebrafish and its Application in the Analysis of Eye Mutants
10:39

Lens Transplantation in Zebrafish and its Application in the Analysis of Eye Mutants

Published on: June 1, 2009

  • Alpha-crystallins increased significantly with zebrafish maturation and aging.
  • Beta-crystallins predominated in young zebrafish lenses, while alpha- and gamma-crystallins increased with age.
  • Novel beta- and gamma-crystallins were identified, and a high molecular weight fraction became dominant by 2.5 years.

    • Conclusions:

    • Zebrafish lens protein expression, particularly crystallins, changes significantly with age.
    • These age-related changes are essential for maintaining lens optical function.
    • Novel crystallins exist within the zebrafish genome.