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Transgenic Organisms

Transgenic Organisms

Transgenic Organisms

Transgenic Organisms

Transgenic Plants

Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...

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Related Experiment Video

Updated: Jun 18, 2026

Generation of Transgenic Rats using a Lentiviral Vector Approach

Generation of Transgenic Rats using a Lentiviral Vector Approach

Published on: May 17, 2020

Animal transgenesis: an overview.

Miguel A Gama Sosa¹, Rita De Gasperi, Gregory A Elder

¹Department of Psychiatry, Mount Sinai School of Medicine, One Gustave L. Levy Place, New York, NY, 10029, USA. miguel.gama-sosa@mssm.edu

Brain Structure & Function

|November 26, 2009

Summary

Transgenic animals are crucial tools for studying gene function and modeling diseases. This review covers established and novel methods for genetic modification in various species, focusing on mice.

Area of Science:

Genetics and Genomics
Animal Models
Biotechnology

Background:

Transgenic animals are vital for in vivo gene function studies and disease modeling.
Mice are the predominant model organism for neurodegenerative disease research.
Established techniques include pronuclear injection and embryonic stem cell (ESC)-mediated gene targeting.

Purpose of the Study:

To comprehensively review strategies for genetic modification in transgenic animals.
To highlight recent methodologies applicable across diverse species.
To discuss techniques for conditional gene knockout and inducible transgene expression.

Main Methods:

Pronuclear injection into fertilized oocytes.
Embryonic stem cell (ESC)-mediated gene targeting for knockouts and subtle modifications.

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Lentiviral Mediated Production of Transgenic Mice: A Simple and Highly Efficient Method for Direct Study of Founders

Lentiviral Mediated Production of Transgenic Mice: A Simple and Highly Efficient Method for Direct Study of Founders

Published on: October 7, 2018

Electroporation-Based Genetic Modification of Primary Human Pigment Epithelial Cells Using the Sleeping Beauty Transposon System

Electroporation-Based Genetic Modification of Primary Human Pigment Epithelial Cells Using the Sleeping Beauty Transposon System

Published on: February 4, 2021

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Last Updated: Jun 18, 2026

Generation of Transgenic Rats using a Lentiviral Vector Approach

Generation of Transgenic Rats using a Lentiviral Vector Approach

Published on: May 17, 2020

Lentiviral Mediated Production of Transgenic Mice: A Simple and Highly Efficient Method for Direct Study of Founders

Lentiviral Mediated Production of Transgenic Mice: A Simple and Highly Efficient Method for Direct Study of Founders

Published on: October 7, 2018

Electroporation-Based Genetic Modification of Primary Human Pigment Epithelial Cells Using the Sleeping Beauty Transposon System

Electroporation-Based Genetic Modification of Primary Human Pigment Epithelial Cells Using the Sleeping Beauty Transposon System

Published on: February 4, 2021

Retrovirus-mediated gene transfer.

RNA interference (RNAi)-mediated gene knockdown.

Somatic cell mutagenesis combined with nuclear transfer.

Main Results:

ESC technology enables precise genetic modifications, including single nucleotide changes.
Conditional and inducible gene manipulation strategies are available.
Emerging methods offer broader applicability for species challenging for traditional techniques.

Conclusions:

A range of techniques exists for creating genetically modified animals, with mice being a primary model.
Advancements in gene transfer and editing offer new possibilities for diverse species.
These methods are essential for advancing biological research and disease modeling.