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Evaluation of API Coryne in comparison with conventional methods for identifying coryneform bacteria
J Freney1, M T Duperron, C Courtier
1Laboratoire de Bactériologie, Faculté de Pharmacie, Lyon, France.
Journal of Clinical Microbiology
|January 1, 1991
Summary
The API Coryne system offers a reliable and rapid method for identifying coryneform bacteria, showing high concordance with conventional techniques. This miniaturized system aids in bacterial identification, though some isolates required additional testing.
Area of Science:
- Microbiology
- Bacteriology
Background:
- Accurate identification of bacterial isolates is crucial in clinical and research settings.
- Conventional biochemical methods can be time-consuming and may not cover all bacterial species effectively.
Purpose of the Study:
- To evaluate the performance of the API Coryne system for the identification of coryneform and related bacteria.
- To compare the API Coryne system's accuracy and speed against conventional biochemical identification methods.
Main Methods:
- A total of 240 bacterial isolates were analyzed using the API Coryne system.
- Conventional biochemical methods were used as a reference for identification.
- Isolates were incubated for 24 and 48 hours to assess identification times.
Main Results:
- The API Coryne system achieved a 97.6% concordance with conventional methods.
- 65.8% of isolates were identified within 24 hours without further testing.
- Only 1.2% of strains were misidentified, and 1.2% could not be identified by the system.
Conclusions:
- The API Coryne system is a reliable and rapid tool for the identification of coryneform bacteria.
- The system demonstrates high accuracy, comparable to conventional methods, with improved efficiency.
- Further investigation may be needed for isolates not covered by the system's database.