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Evaluation of the Staph-Zym system with staphylococci isolated from bovine intramammary infections
1Mastitis Research Laboratory, Louisiana State University Agricultural Center Homer 71040.
Abstract:
A total of 148 staphylococci isolated from bovine intramammary infections were used to evaluate the Staph-Zym system (ROSCO, Taastrup, Denmark). The overall accuracy of the system was 91.9%. The system correctly identified all strains of Staphylococcus aureus, Staphylococcus simulans, and Staphylococcus xylosus and 95% of Staphylococcus intermedius strains. Of 33 Staphylococcus hyicus strains, 31 (93.9%) were classified correctly by the Staph-Zym system, as well as 8 (80%) of 10 Staphylococcus chromogenes strains. All 11 Staphylococcus epidermidis strains and the 1 Staphylococcus haemolyticus strain included in the study were identified, but the Staph-Zym system had difficulty distinguishing strains of Staphylococcus warneri and Staphylococcus hominis from other species in the S. epidermidis group. The Staph-Zym system correctly identified all six S. xylosus strains and two of three Staphyloccus sciuri strains. The Staph-Zym system was considered an acceptable alternative to conventional methods for identification of bovine mammary gland isolates.
Insights
The Staph-Zym system accurately identifies staphylococci from bovine intramammary infections, showing 91.9% overall accuracy. This rapid identification method is a viable alternative to traditional techniques for dairy cattle mastitis diagnostics.
Area of Science:
- Veterinary Microbiology
- Bacteriology
- Diagnostic Microbiology
Background:
- Bovine intramammary infections (IMIs) are frequently caused by staphylococcal species.
- Accurate and rapid identification of staphylococci is crucial for effective treatment and herd management.
- Conventional identification methods can be time-consuming and labor-intensive.
Purpose of the Study:
- To evaluate the diagnostic accuracy of the Staph-Zym system for identifying staphylococcal isolates from bovine mastitis.
- To compare the performance of the Staph-Zym system against conventional identification methods.
Main Methods:
- A total of 148 staphylococcal isolates from bovine IMIs were analyzed.
- The Staph-Zym system was used for the biochemical identification of the isolates.
- Results were compared with established identification criteria.
Main Results:
- The Staph-Zym system achieved an overall accuracy of 91.9% for the tested isolates.
- High accuracy was observed for Staphylococcus aureus, S. simulans, S. xylosus, S. intermedius, and S. hyicus.
- The system showed some limitations in differentiating species within the Staphylococcus epidermidis group, including S. warneri and S. hominis.
Conclusions:
- The Staph-Zym system is a reliable and accurate tool for the identification of common staphylococcal species causing bovine mastitis.
- It offers an acceptable alternative to conventional methods, potentially improving diagnostic efficiency in veterinary laboratories.