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Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Jun 18, 2026

Discrimination of Seven Immune Cell Subsets by Two-fluorochrome Flow Cytometry
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Published on: March 5, 2019

Data File Standard for Flow Cytometry, version FCS 3.1.

Josef Spidlen1, Wayne Moore, David Parks

  • 1Terry Fox Laboratory, BC Cancer Agency, Vancouver, British Columbia, Canada.

Cytometry. Part a : the Journal of the International Society for Analytical Cytology
|November 26, 2009
PubMed
Summary
This summary is machine-generated.

The Flow Cytometry Standard (FCS) 3.1 offers an updated file format for flow cytometry data. This revision enhances data compatibility and robustness across different systems.

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Area of Science:

  • Biotechnology
  • Bioinformatics
  • Data Standards

Background:

  • The Flow Cytometry Standard (FCS) format has evolved since 1984 to standardize flow cytometry data files.
  • Previous versions (FCS 1.0, 2.0, 3.0) aimed to ensure data compatibility across diverse hardware and software.
  • Community feedback highlighted areas for improvement in existing standards.

Purpose of the Study:

  • To introduce the next generation of the Flow Cytometry Standard, designated as FCS 3.1.
  • To provide a uniform file format for flow cytometry data, ensuring interoperability between different acquisition systems.
  • To address ambiguities and enhance the robustness of the data file standard.

Main Methods:

  • The FCS 3.1 standard retains the core structure and features of previous versions.
  • Incorporates community-suggested improvements and minor revisions.
  • Focuses on refining existing specifications and adding new functionalities.

Main Results:

  • FCS 3.1 introduces simplified international character support and improved compensation storage.
  • Key additions include support for preferred display scale, standardized sample volume capture, and data originality information.
  • Enhanced support for plate and well identification in high-throughput experiments is now available.

Conclusions:

  • FCS 3.1 represents a significant advancement in standardizing flow cytometry data.
  • The updated standard enhances data integrity, interoperability, and usability, particularly for high-throughput applications.
  • This revision ensures continued compatibility and facilitates more robust data analysis in flow cytometry.