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Stimulation of glucose oxidase with white linearly polarized light
Anna Konieczna-Molenda1, Maciej Fiedorowicz, Przemysław J Tomasik
1Dept. of Chemistry, Agricultural University, Krakow 30149, Poland. akonieczna-molenda@ar.krakow.pl
Biotechnology Progress
|November 26, 2009
Abstract:
Glucose oxidase (GOD) was illuminated with white linearly polarized light (WLPL). The enzyme was illuminated at room temperature in separate vessels then admixed to a reactor filled with D-glucose. The illumination of the enzyme for 60 min at 25-30 degrees C and pH 6.5-7.0 provided its superior stimulation as proven in the oxidation of beta-D-glucose. Lyophilization of the illuminated enzyme reduced its activity by, approximately, 30%.
