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Production of Alcohol

Continuous fermentation is a key strategy in industrial ethanol production, particularly when efficiency, scalability, and high yields are essential. This approach allows for uninterrupted operation and optimized resource utilization. The primary feedstock, corn starch, undergoes enzymatic hydrolysis facilitated by α-amylase and glucoamylase. These enzymes break down the starch into fermentable sugars such as glucose, which are readily assimilated by fermentative microorganisms.Fermentation...
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Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol
14:53

Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol

Published on: October 24, 2016

[Consecutive very-high-gravity batch ethanol fermentation with self-flocculation yeast].

Fan Li1, Xumeng Ge, Ning Li

  • 1Department of Bioscience and Bioengineering, Dalian University of Technology, Dalian 116023, China.

Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology
|November 27, 2009
PubMed
Summary
This summary is machine-generated.

To improve ethanol production efficiency, researchers developed a yeast flocculation method. Purging yeast stabilized floc size, ensuring reliable fermentation over extended periods.

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Last Updated: Jun 18, 2026

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Flow Cytometry-based Purification of S. cerevisiae Zygotes
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Flow Cytometry-based Purification of S. cerevisiae Zygotes

Published on: September 21, 2012

Area of Science:

  • Biotechnology
  • Industrial Microbiology
  • Fermentation Technology

Background:

  • Consecutive very-high-gravity batch fermentation offers energy savings for downstream processes.
  • Self-flocculating yeast Saccharomyces cerevisiae flo enables efficient ethanol production.

Purpose of the Study:

  • To address yeast floc sedimentation deterioration in prolonged consecutive fermentation.
  • To maintain yeast viability and fermentation system stability.

Main Methods:

  • Implementing a yeast purging strategy at the end of each batch.
  • Maintaining a low yeast floc concentration to stimulate propagation.
  • Monitoring yeast floc size and sedimentation performance.

Main Results:

  • Initial high ethanol productivity of 8.2 g/(L x h) at 120 g/L ethanol concentration.
  • Deterioration of yeast floc sedimentation observed in prolonged fermentation.
  • Yeast purging stabilized floc size, enabling reliable operation for 14 additional batches.
  • Ethanol productivity decreased to 4.0 g/(L x h) with the purging strategy.

Conclusions:

  • Yeast floc size decrease is the primary cause of sedimentation issues.
  • Stimulating yeast floc propagation through controlled purging enhances system reliability.
  • The modified fermentation system ensures stable operation despite reduced initial productivity.