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[Determination of militarine in Rhizoma Bletillae by HPLC]
Xun He1, Aimin Wang, Yongjun Li
1School of Pharmacy, Guiyang Medical College, Guiyang 550004, China.
Objective:
To develop an HPLC method for determination of militarine in Rhizoma Bletillae.
Method:
Analysis was carried out on a Symmetry C18 analytical column, and a C18 guard column eluted with mobile phase consisted of acetonitrile and water (37:63) militarine the detection wavelength was set at 244 nm.
Result:
The contents of in 12 samples of Rhizoma Bletillae in market were in the ranges of 1.45%-3.32%.
Conclusion:
The method is simple, accurate and reproducible, and is suitable for the quality control of Rhizoma Bletillae.
