Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 18, 2026

CD4+ T-Lymphocyte Capture Using a Disposable Microfluidic Chip for HIV
12:44

CD4+ T-Lymphocyte Capture Using a Disposable Microfluidic Chip for HIV

Published on: October 1, 2007

Microfluidic CD4+ T-cell counting device using chemiluminescence-based detection.

Zuankai Wang, Sau Yin Chin, Curtis D Chin

    Analytical Chemistry
    |November 27, 2009
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    High-Performance Weavable Piezoelectret Fibers via Scalable In Situ Poling Melt-Spinning for Real-Time Knee Joint Rehabilitation Monitoring.

    Advanced materials (Deerfield Beach, Fla.)·2026
    Same author

    Reverse Tesla valve modulated efficient water evaporation and cooling.

    Nature communications·2026
    Same author

    A Paintable Bioinspired Stratified Skin Resolving the Cooling-Electricity Trade-Off for All-Weather Building Retrofits.

    Advanced materials (Deerfield Beach, Fla.)·2026
    Same author

    Pre-Exposure Prophylaxis Adherence and HIV Self-Testing App Among Women in the South Bronx: 12-Month Usability, Acceptability, and Feasibility Study.

    JMIR formative research·2026
    Same author

    Orbital Electrowetting: From Continuous Droplet Transport to Programmable Microfluidics.

    Advanced materials (Deerfield Beach, Fla.)·2026
    Same author

    Orchestrated Multiscale Effects Boost Performances of Hybrid Carbon-Based Water-Electricity Cogenerators.

    ACS nano·2026
    Same journal

    The ACS at 150: The History of Analytical Chemistry Publications and a Century of Progress.

    Analytical chemistry·2026
    Same journal

    Machine Learning-Enabled Image Analysis of Complex Chemical Mixtures: Synthetic Urine Droplets as a Test System.

    Analytical chemistry·2026
    Same journal

    H<sub>2</sub>O<sub>2</sub>/Viscosity Tandem-Locked Fluorescent Probes Based on an In Situ Fluorophore Synthesis Strategy for Colitis Imaging and Diagnosis.

    Analytical chemistry·2026
    Same journal

    TopoStitcher: A Geometric-Topological Structure-Guided Stitching Framework for Single-Molecule Localization Microscopy.

    Analytical chemistry·2026
    Same journal

    Noninvasive SERS Immunosensing of Tyrosinase for Melanoma Monitoring via Microneedle Sampling Integrated with Satellite-Structured Bifunctional Nanozymes.

    Analytical chemistry·2026
    Same journal

    Label-Free Electrochemical CRISPR Platform Gated by Allosteric Transcription Factors for Ultrasensitive Small-Molecule Detection.

    Analytical chemistry·2026
    See all related articles

    This study presents a microfluidic device for counting CD4+ T-lymphocytes in whole blood using chemiluminescence. The platform offers rapid, accurate CD4 cell counts comparable to flow cytometry, with simplified operation.

    Area of Science:

    • Biomedical Engineering
    • Immunology
    • Analytical Chemistry

    Background:

    • Accurate enumeration of CD4+ T-lymphocytes is critical for monitoring immune status, particularly in HIV/AIDS management.
    • Existing methods like flow cytometry can be complex, expensive, and require specialized facilities.
    • Development of rapid, portable, and cost-effective CD4 counting platforms is essential for widespread clinical application.

    Discussion:

    • A novel microfluidic platform was developed for CD4+ T-lymphocyte enumeration using anti-CD4 antibody-coated microtraps.
    • Chemiluminescence detection, based on horseradish peroxidase-conjugated CD3 antibodies, provides a signal proportional to captured CD4+ T-cells.
    • The system analyzes small volumes of whole blood (3 µL) with high capture efficiency and correlation to flow cytometry.

    Key Insights:

    More Related Videos

    Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays
    09:58

    Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays

    Published on: June 23, 2022

    Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood
    08:58

    Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood

    Published on: April 16, 2016

    Related Experiment Videos

    Last Updated: Jun 18, 2026

    CD4+ T-Lymphocyte Capture Using a Disposable Microfluidic Chip for HIV
    12:44

    CD4+ T-Lymphocyte Capture Using a Disposable Microfluidic Chip for HIV

    Published on: October 1, 2007

    Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays
    09:58

    Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays

    Published on: June 23, 2022

    Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood
    08:58

    Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood

    Published on: April 16, 2016

    • The microfluidic device achieves high CD4+ T-cell capture efficiency and accurate quantification.
    • Chemiluminescence detection eliminates the need for external light sources and image processing.
    • Results are obtained digitally within seconds, simplifying the diagnostic workflow.

    Outlook:

    • This technology has the potential to serve as a point-of-care diagnostic tool for immune monitoring.
    • Further optimization could lead to integration with other biomarkers for comprehensive immune profiling.
    • The platform's simplicity and speed can improve accessibility to CD4+ T-cell counts in resource-limited settings.