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Three-dimensional organization of the Golgi complex observed by scanning electron microscopy
1Department of Anatomy, Tottori University School of Medicine, Yonago, Japan.
Journal of Electron Microscopy Technique
|January 1, 1991
Summary
Scanning electron microscopy reveals the Golgi complex
Area of Science:
- Cell Biology
- Microscopy Techniques
Background:
- The Golgi complex's 3D structure was challenging to study.
- Advancements in specimen preparation and SEM resolution are key.
Purpose of the Study:
- To investigate the three-dimensional organization of the Golgi complex using high-resolution SEM.
- To detail the morphology of Golgi cisternae and their connections.
Main Methods:
- High-resolution scanning electron microscopy (SEM).
- Osmium-DMSO-osmium specimen preparation technique.
Main Results:
- Cis-cisternae appear as sieve-like plates with perforations.
- Trans-cisternae exhibit fenestrated or reticular patterns.
- Distinctive trans-side structures and inter-cisternal connections were observed.
- Connections between Golgi and rough endoplasmic reticulum (ER) were visualized.
Conclusions:
- High-resolution SEM effectively visualizes Golgi complex 3D organization.
- Specific morphological features of Golgi cisternae were identified.
- Interconnections within and between Golgi stacks, and with the ER, were detailed.