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Published on: April 25, 2014
Mycobacterium tuberculosis spoligotypes in Monterrey, Mexico
Carmen A Molina-Torres1, Elisa Moreno-Torres, Jorge Ocampo-Candiani
1Servicio de Dermatología, Hospital Universitario José E. González, Madero y Gonzalitos, Col. Mitras Centro, Monterrey, N.L. CP 64460 Mexico.
Journal of Clinical Microbiology
|November 27, 2009
Summary
This study reveals genetic diversity in Mycobacterium tuberculosis isolates from Monterrey, Mexico. SIT53 and SIT119 spoligotypes are predominant, with distinct age and gender distributions observed in tuberculosis patients.
Area of Science:
- Microbiology
- Genetics
- Epidemiology
Background:
- Tuberculosis remains a significant public health issue in Mexico.
- Limited data exists on the genetic profiles of Mycobacterium tuberculosis isolates within the country.
Purpose of the Study:
- To characterize the genetic diversity of Mycobacterium tuberculosis clinical isolates from Monterrey, Mexico.
- To identify predominant spoligotypes and their distribution among drug-susceptible and drug-resistant strains.
Main Methods:
- Spoligotyping was performed on 180 Mycobacterium tuberculosis clinical isolates.
- Patterns were compared against the international SITVIT2 database.
- IS6110-based restriction fragment length polymorphism (RFLP) and gene mutation analysis (katG, rpoB) were used for drug-resistant isolates.
Main Results:
- 44 distinct spoligo international types (SITs) were identified among the 180 isolates.
- SIT53 (clade T1) and SIT119 (clade X1) were the most prevalent, accounting for 23.8% and 15.5% of isolates, respectively.
- SIT119 showed a predilection for younger males (0-20 years), while SIT53 was more common in older females.
Conclusions:
- Significant genetic variability exists among Mycobacterium tuberculosis isolates in Monterrey.
- SIT53 and SIT119 are the dominant spoligotypes in this region of Mexico.
- Distinct epidemiological patterns were observed for prevalent spoligotypes based on patient age and sex.
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