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Enhanced detection of enteroviruses in clinical samples by reverse transcription-PCR using complementary locked

JiYoung Hong1, Byunghak Kang, Ahyoun Kim

  • 1Division of Enteric and Hepatitis Viruses, Center for Infectious Diseases, National Institute of Health, 5 Nokbun-dong, Eunpyung-gu, Seoul 122-701, South Korea.

Journal of Clinical Microbiology
|November 27, 2009
PubMed

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Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...