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Production of Antibiotics01:27

Production of Antibiotics

Penicillin, one of the earliest and most widely used antibiotics, is produced industrially by the filamentous fungus Penicillium chrysogenum. Large stirred-tank bioreactors ranging from tens to hundreds of thousands of liters maintain tightly controlled temperature, pH, and dissolved oxygen conditions to support fungal metabolism and maximize antibiotic yield. Penicillin is a secondary metabolite, synthesized primarily during the stationary growth phase, which requires a carefully managed...

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Purification of a High Molecular Mass Protein in Streptococcus mutans
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Rapid method for extracting the antibiotic mutacin 1140 from complex fermentation medium yeast extract.

Noel Chaney1, Shawanda Wilson-Stanford, James Kastrantas

  • 1Mississippi State University, Department of Biological Sciences at the College of Veterinary Medicine, Mississippi State, MS 39762, USA.

Canadian Journal of Microbiology
|November 27, 2009
PubMed
Summary

An efficient ammonium sulfate precipitation method was developed to purify the lantibiotic mutacin 1140. This method simplifies downstream processing and improves yield from complex media.

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Area of Science:

  • Microbiology
  • Biochemistry
  • Analytical Chemistry

Background:

  • Lantibiotics, such as mutacin 1140, are ribosomally synthesized peptide antibiotics.
  • These compounds undergo complex post-translational modifications.
  • Efficient purification is crucial for studying and utilizing lantibiotics.

Purpose of the Study:

  • To develop an efficient method for purifying mutacin 1140 from complex fermentation media.
  • To minimize downstream purification challenges and costs.
  • To assess the adaptability of the method for other lantibiotics.

Main Methods:

  • Utilized reverse-phase-high-performance liquid chromatography (RP-HPLC) for tracking mutacin 1140.
  • Employed a semiquantitative bacteriocin bioassay for quantification.
  • Developed an ammonium sulfate (AS) precipitation technique.

Main Results:

  • An efficient AS precipitation method was established for mutacin 1140 purification.
  • The method effectively reduced fermentation by-products and media components.
  • A threefold decrease in yeast extract precipitation was observed at pH 8.0 compared to pH 2.0.
  • The average yield of mutacin 1140 was 66%.

Conclusions:

  • The developed AS precipitation method is effective for initial purification of mutacin 1140.
  • This technique can simplify downstream processing and enhance economic feasibility.
  • The method shows potential for purifying other lantibiotics and secondary metabolites from complex media.