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Published on: September 19, 2013
Isolation of cutaneous dendritic cells
1Department of Gene and Cell Medicine and The Immunology Institute, Mount Sinai School of Medicine, New York, NY, USA.
Methods in Molecular Biology (Clifton, N.J.)
|November 27, 2009
Summary
Isolate cutaneous dendritic cells from mouse ears for immunological studies. This protocol enables flow cytometry and functional assays for skin defense research.
Area of Science:
- Immunology
- Cell Biology
Background:
- Cutaneous dendritic cells are critical immune cells at the skin's surface.
- They act as the first line of defense against invading pathogens.
- Understanding their function is key to developing new treatments for skin infections.
Purpose of the Study:
- To provide a detailed protocol for isolating cutaneous dendritic cells from mouse ears.
- To enable subsequent flow cytometry analysis and functional assays.
- To facilitate research on skin immunity and host defense mechanisms.
Main Methods:
- Isolation of dendritic cells from murine ear tissue.
- Preparation of single-cell suspensions.
- Utilizing flow cytometry for cell characterization.
- Performing functional assays to assess cell activity.
Main Results:
- Successfully isolated viable cutaneous dendritic cells from mouse ear samples.
- Demonstrated the utility of the isolated cells for flow cytometry.
- Confirmed the suitability of the protocol for functional assays.
Conclusions:
- This protocol provides a reliable method for obtaining cutaneous dendritic cells from mouse ears.
- The isolated cells are suitable for advanced immunological analyses.
- This technique will aid further investigation into skin-associated immune responses.
