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Related Experiment Video

Updated: Jun 18, 2026

Analysis of Pulmonary Dendritic Cell Maturation and Migration during Allergic Airway Inflammation
07:52

Analysis of Pulmonary Dendritic Cell Maturation and Migration during Allergic Airway Inflammation

Published on: July 23, 2012

Methods to study pulmonary dendritic cell migration.

Claudia Jakubzick1, Gwendalyn J Randolph

  • 1Department of Gene and Cell Medicine, Icahn Research Institute, Mount Sinai School of Medicine, New York, NY, USA.

Methods in Molecular Biology (Clifton, N.J.)
|November 27, 2009
PubMed
Summary
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This study presents a protocol to track pulmonary dendritic cell migration from airways to lymph nodes without inflammation, using fluorescent OVA or latex beads for allergy and immunity research.

Area of Science:

  • Immunology
  • Cell Biology
  • Respiratory Medicine

Background:

  • Dendritic cell migration is crucial for airway immunity during infection, allergy, and vaccination.
  • Understanding this migration is key for developing effective immunotherapies and vaccines.
  • Current methods often involve inflammation, complicating the study of baseline migration.

Purpose of the Study:

  • To describe a protocol for studying dendritic cell migration to mediastinal lymph nodes without inducing inflammation.
  • To provide methods for labeling and tracking pulmonary dendritic cells from the airway and lung.
  • To identify and mitigate potential pitfalls in dendritic cell migration assays.

Main Methods:

  • Airway administration of fluorescent ovalbumin (OVA) or latex beads.

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Last Updated: Jun 18, 2026

Analysis of Pulmonary Dendritic Cell Maturation and Migration during Allergic Airway Inflammation
07:52

Analysis of Pulmonary Dendritic Cell Maturation and Migration during Allergic Airway Inflammation

Published on: July 23, 2012

Precision-cut Mouse Lung Slices to Visualize Live Pulmonary Dendritic Cells
09:33

Precision-cut Mouse Lung Slices to Visualize Live Pulmonary Dendritic Cells

Published on: April 5, 2017

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Study of Cell Migration in Microfabricated Channels

Published on: February 21, 2014

  • Labeling and tracking of pulmonary dendritic cells.
  • Analysis of cell migration to mediastinal lymph nodes.

    • Main Results:

    • Successful tracking of dendritic cells from the airway and lung to mediastinal lymph nodes.
    • Demonstration of two distinct, non-inflammatory methods (fluorescent OVA and latex beads) for studying migration.
    • Identification of critical steps and potential pitfalls to ensure assay validity.

    Conclusions:

    • The described protocol enables robust, non-inflammatory assessment of dendritic cell migration.
    • This method is valuable for research in airway immunity, allergy, and vaccine development.
    • Optimized assays improve the reliability of studying pulmonary immune responses.