Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Non-sebaceous lymphadenoma of the salivary gland: case report with immunohistochemical investigation.

Virchows Archiv : an international journal of pathology·2007
Same author

[Enhancement of HSP-MUC1 antitumor activity by type C CpG-ODN BW005].

Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology·2007
Same author

Inhibition of severe acute respiratory syndrome-associated coronavirus infection by equine neutralizing antibody in golden Syrian hamsters.

Viral immunology·2007
Same author

Synergistic effect between components of mixtures of cationic amphipaths in transfection of primary endothelial cells.

Molecular pharmaceutics·2007
Same author

The insertion polymorphism in angiotensin-converting enzyme gene associated with the APOE epsilon 4 allele increases the risk of late-onset Alzheimer disease.

Journal of molecular neuroscience : MN·2007
Same author

[Study on the analysis of mixed spectra of benzene homologs with Dolittle multivariate correction method].

Guang pu xue yu guang pu fen xi = Guang pu·2007
Same journal

Mapping the 3D Chromosome Organization of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of Streptomyces by Hi-C.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

CUT&Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Characterization of Bioactive Saponins from Sea Cucumbers.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for Functional Validation of Terpenoid Metabolic Clusters in Nicotiana benthamiana and Aspergillus oryzae.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: Jun 18, 2026

A Simple and Efficient Method for Testing Immunomodulatory Agents for Generation of Tolerogenic Dendritic Cells from Human CD14+ Monocytes
11:34

A Simple and Efficient Method for Testing Immunomodulatory Agents for Generation of Tolerogenic Dendritic Cells from Human CD14+ Monocytes

Published on: April 11, 2025

Adaptive Treg generation by DCs and their functional analysis.

Li Wang1

  • 1Dartmouth Medical School, Norris Cotton Cancer Center, Lebanon, NH, USA.

Methods in Molecular Biology (Clifton, N.J.)
|November 27, 2009
PubMed
Summary
This summary is machine-generated.

This study details methods for converting naive CD4 T cells into adaptive regulatory T cells (aTregs) using ex vivo splenic dendritic cells and TGF-beta. This approach is key for immune tolerance induction.

More Related Videos

Generation of Induced Regulatory T Cells from Primary Human Naïve and Memory T Cells
14:23

Generation of Induced Regulatory T Cells from Primary Human Naïve and Memory T Cells

Published on: April 16, 2012

Adenoviral Transduction of Naive CD4 T Cells to Study Treg Differentiation
15:33

Adenoviral Transduction of Naive CD4 T Cells to Study Treg Differentiation

Published on: August 13, 2013

Related Experiment Videos

Last Updated: Jun 18, 2026

A Simple and Efficient Method for Testing Immunomodulatory Agents for Generation of Tolerogenic Dendritic Cells from Human CD14+ Monocytes
11:34

A Simple and Efficient Method for Testing Immunomodulatory Agents for Generation of Tolerogenic Dendritic Cells from Human CD14+ Monocytes

Published on: April 11, 2025

Generation of Induced Regulatory T Cells from Primary Human Naïve and Memory T Cells
14:23

Generation of Induced Regulatory T Cells from Primary Human Naïve and Memory T Cells

Published on: April 16, 2012

Adenoviral Transduction of Naive CD4 T Cells to Study Treg Differentiation
15:33

Adenoviral Transduction of Naive CD4 T Cells to Study Treg Differentiation

Published on: August 13, 2013

Area of Science:

  • Immunology
  • Cell Biology
  • T cell differentiation

Background:

  • Naturally occurring thymus-derived CD4(+)CD25(+)Foxp3(+) regulatory T cells (nTregs) are crucial for maintaining immune tolerance.
  • Naive CD4 T cells can be converted into Foxp3(+) regulatory T cells (Tregs) both in vivo and ex vivo.

Purpose of the Study:

  • To summarize methods for inducing adaptive Foxp3(+)CD4(+) regulatory T cells (aTregs) from naive CD4 T cells.
  • To explore the role of ex vivo splenic dendritic cells (DCs) in this conversion process.

Main Methods:

  • Utilizing ex vivo splenic DCs to stimulate naive CD4 T cells.
  • Employing transforming growth factor-beta (TGF-beta) and suboptimal T cell receptor (TCR) stimulation.
  • Controlling co-stimulatory signals from antigen-presenting cells.

Main Results:

  • Demonstrated that immature, steady-state DCs contribute to immune tolerance, unlike mature DCs which initiate adaptive immunity.
  • Established protocols for converting naive CD4 T cells into aTregs ex vivo.

Conclusions:

  • Ex vivo splenic DCs can be effectively used to induce aTregs.
  • Understanding DC co-stimulation is vital for regulating T cell conversion and immune tolerance.