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Isolation and Characterization of Extracellular Vesicles from Adult Schistosoma japonicum

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Do schistosomes grow old? A confocal laser scanning microscopy study.

J R Machado-Silva¹, R H Neves, R Rodrigues-Silva

¹Laboratório de Hemintologia Romero Lascasas Porto, Departamento de Microbiologia, Imunologia e Parasitologia, Faculdade de Ciências Médicas, Universidade do Estado do Rio de Janeiro, 20511- 070 Rio de Janeiro, RJ, Brasil. machado@uerj.br

Journal of Helminthology

|November 28, 2009

Summary

Ageing causes infertility in older Schistosoma mansoni worms, leading to decreased egg production. This study observed morphological changes in aged worms, indicating impaired reproductive function.

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Area of Science:

Parasitology
Helminthology
Reproductive Biology

Background:

Schistosoma mansoni infection leads to reduced egg output in hosts during chronic phases.
Ageing is a potential factor contributing to decreased reproductive capacity in schistosomes.

Purpose of the Study:

To investigate the effects of ageing on the morphology of adult Schistosoma mansoni worms.
To understand the mechanisms behind age-related infertility in schistosomes.

Main Methods:

Confocal laser scanning microscopy was used to examine adult Schistosoma mansoni worms.
Worms were recovered from experimentally infected Nectomys squamipes (water rat) after prolonged infection periods.

Main Results:

While some reproductive organs (ovary, vitellaria, oocytes) appeared normal, the ootype showed signs of impaired eggshell production.
Male worms exhibited empty areas in testicular lobes and flaccid dorsal musculature.
Infected rats passed viable eggs for 65 weeks, followed by unviable eggs until death at 70 weeks.

Conclusions:

Older male and female Schistosoma mansoni worms display morphological changes indicative of infertility.
Ageing significantly impacts the reproductive capabilities of schistosomes, affecting egg viability and production.