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Related Concept Videos

DNA Helicases00:55

DNA Helicases

DNA unwinding helicase enzymes are a type of motor protein. Motor proteins can translocate along filaments or polymers using energy generated from ATP hydrolysis. Helicases are involved in all the important cellular processes where DNA unwinding is required, such as DNA replication, repair, recombination, and transcription. They are present in all living organisms, but vary in their structure, function, and mechanism of action. For example, in prokaryotes, DnaB helicase binds and translocates...
The Replisome03:01

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DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
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The Replisome03:01

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DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
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Coordination of Gene Expression Processes in Bacteria

The DNA replication, transcription, and translation processes are intricately coupled in bacteria, allowing efficient gene expression and rapid protein synthesis. While this physical and functional coordination is advantageous, it introduces challenges that bacteria overcome through specific regulatory mechanisms.Coupling of Replication, Transcription, and TranslationThe coupling of replication, transcription, and translation is a hallmark of bacterial gene expression. As the replisome unwinds...

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Hybrid Ensemble and Single-molecule Assay to Image the Motion of Fully Reconstituted CMG
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Helicase multitasking in ribosome assembly.

Ulf-Peter Guenther1, Eckhard Jankowsky

  • 1Department of Biochemistry, Case Western Reserve University, Cleveland, OH 44106, USA.

Molecular Cell
|November 28, 2009
PubMed
Summary

Researchers found the RNA helicase Prp43 binds to multiple sites on preribosomal RNA. These locations suggest Prp43 plays distinct roles in ribosome biogenesis.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • Ribosome biogenesis is a complex cellular process essential for protein synthesis.
  • RNA helicases are crucial enzymes involved in RNA metabolism and cellular processes.
  • Prp43 is an RNA helicase implicated in various RNA processing events.

Purpose of the Study:

  • To identify and characterize the binding sites of the RNA helicase Prp43 on preribosomal RNA.
  • To elucidate the functional implications of Prp43 binding to specific regions of preribosomal RNA.

Main Methods:

  • The study employed biochemical methods to map the binding sites of Prp43 on preribosomal RNA.
  • Analysis of target regions was performed to infer functional roles.

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Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase

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Last Updated: Jun 18, 2026

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Main Results:

  • Multiple distinct binding sites for the RNA helicase Prp43 on preribosomal RNA were identified.
  • The identified target regions suggest specialized functions for Prp43 during ribosome biogenesis.

Conclusions:

  • Prp43 interacts with specific regions of preribosomal RNA, indicating diverse roles in ribosome assembly.
  • Understanding Prp43 binding sites provides insights into the regulation of ribosome biogenesis.