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Preparation and In Vitro Characterization of Magnetized miR-modified Endothelial Cells
Published on: May 2, 2017
Genetically engineered angiogenic cell sheets using magnetic force-based gene delivery and tissue fabrication
Hirokazu Akiyama1, Akira Ito, Yoshinori Kawabe
1Department of Chemical Engineering, Faculty of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan.
Biomaterials
|November 28, 2009
Summary
Researchers developed a new method for tissue engineering using magnetic nanoparticles to create vascularized cell sheets. This technique enhances blood vessel formation, leading to thicker tissues and improved cell density in grafts.
Area of Science:
- Tissue Engineering
- Biotechnology
- Regenerative Medicine
Background:
- Insufficient vascularization is a key limitation in tissue engineering, hindering cell density and graft size.
- Developing methods to promote blood vessel formation in engineered tissues is crucial for successful implantation.
Purpose of the Study:
- To fabricate angiogenic cell sheets using magnetic force-based techniques.
- To enhance gene delivery and cell accumulation for improved tissue construct development.
Main Methods:
- Utilized magnetite cationic liposomes (MCLs) for magnetofection to deliver vascular endothelial growth factor (VEGF) genes to C2C12 cells.
- Employed magnetic cell accumulation to form multilayered cell sheets from MCL-labeled cells.
- Transplanted VEGF gene-engineered C2C12 cell sheets into nude mice for in vivo evaluation.
Main Results:
- MCL-mediated gene delivery increased transduction efficiency by 6.7-fold compared to conventional methods.
- Constructed cell sheets demonstrated spontaneous formation of multilayered structures.
- Subcutaneous grafts of VEGF-engineered cell sheets showed significant vascularization and high cell density by day 14.
Conclusions:
- The combined use of magnetofection and magnetic cell accumulation with MCLs is a powerful strategy for fabricating angiogenic cell sheets.
- This method effectively promotes vascularization and tissue formation, addressing a major challenge in tissue engineering.
- The developed technique holds significant potential for advancing regenerative medicine applications.
