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Updated: Jun 18, 2026

Continuously-stirred Anaerobic Digester to Convert Organic Wastes into Biogas: System Setup and Basic Operation
Published on: July 13, 2012
Effect of micro-aeration and leachate replacement on COD solubilization and VFA production during mono-digestion of
Padma Shanthi Jagadabhi1, Prasad Kaparaju, Jukka Rintala
1University of Jyväskylä, Department of Biological and Environmental Science, P.O. Box 35, FI-40014 Jyväskylä, Finland. pasjaga@jyu.fi
Abstract:
The effect of micro-aeration and leachate replacement with fresh water on chemical oxygen demand (COD) solubilization and volatile fatty acid (VFA) production during the mono-digestion of grass-silage in one-stage leach-bed reactors (LBRs) was investigated in four LBRs, L0 (control), L1, L2 and L3 in batch mode at 35 + or - 1 degrees Celsius for 57 days. Results showed that leachate replacement without pH adjustment (L3) resulted in 2.7 and 1.3 times more SCOD in the leachate compared to control (L0) or leachate replacement with initial pH adjustment (L1), respectively. Micro-aeration at flow rate of 1 L min(-1) (2.5 L of air) in L2 resulted in 4-fold increase in VFA production (from 2.2 to 9 g L(-1)) without any significant increase in cumulative SCOD in the leachate. Increasing the air flow rate to 4 L min(-1) (24 L of air) in L2 resulted in a decrease in SCOD extraction. Leachate replacement without pH adjustment (L3) resulted in higher (mean) specific SCOD production (0.51 g SCOD g(-1) VS(added)) than control (L0, 0.34 g SCOD g(-1) VS(added)), leachate replacement with initial pH adjustment (L1, 0.33 g SCOD g(-1) VS(added)) or micro-aeration (L2, 0.32 g SCOD g(-1) VS(added)). These results suggest that the challenge of hydrolysis during anaerobic digestion of particulate substrates like grass-silage can be improved by micro-aeration and leachate replacement methods with or without pH adjustment.
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