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Related Experiment Video

Updated: Jun 18, 2026

Constructing Mutants in Serotype 1 Streptococcus pneumoniae strain 519/43
06:06

Constructing Mutants in Serotype 1 Streptococcus pneumoniae strain 519/43

Published on: September 11, 2020

Polymorphic competence peptides do not restrict recombination in Streptococcus pneumoniae.

Omar E Cornejo1, Lesley McGee, Daniel E Rozen

  • 1Department of Biology, Emory University, Program in Population Biology, Ecology, and Evolution, USA. ocornejo@gmail.com

Molecular Biology and Evolution
|November 28, 2009
PubMed
Summary

Polymorphism in Streptococcus pneumoniae

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Area of Science:

  • Bacterial genetics
  • Population genetics
  • Microbial evolution

Background:

  • Bacterial recombination is crucial for genetic variation.
  • Streptococcus pneumoniae competence (com) apparatus is polymorphic.
  • Pherotypes may generate subpopulation genetic structure.

Purpose of the Study:

  • To test if pherotypes limit recombination in S. pneumoniae.
  • To investigate genetic differentiation between pherotype subgroups.
  • To understand the role of competence polymorphism.

Main Methods:

  • Sequenced the competence-inducing peptide locus (comC).
  • Assigned clones to pherotype classes.
  • Used multilocus sequence typing and coalescent analysis.

Main Results:

  • Two dominant pherotypes (CSP1, CSP2) found at high frequencies.
  • No significant genetic differentiation detected between pherotype groups.
  • Strong evidence for interpherotype recombination.
  • Positive selection may maintain comC polymorphism.

Conclusions:

  • Results do not support pherotypes limiting recombination in S. pneumoniae.
  • The competence system polymorphism may not structure populations as predicted.
  • Discussed reasons for expected results and evolution of competence polymorphism.