Related Experiment Video
Updated: Jun 18, 2026

Paraffin Embedding and Thin Sectioning of Microbial Colony Biofilms for Microscopic Analysis
Published on: March 23, 2018
The value of the reprocessing method of paraffin-embedded biopsies for transmission electron microscopy
Rodica Lighezan1, Flavia Baderca, Aurora Alexa
1Department of Histology, "Victor Babes" University of Medicine and Pharmacy, Timisoara, Romania. rodalighezan@yahoo.com
Abstract:
Transmission electron microscopy (TEM) implies an elaborate preparation protocol that includes: fixation in glutaraldehyde followed by osmium tetraoxide postfixation, specimen dehydration, infiltration, resin embedding, ultrathin sectioning and staining with heavy metal salts. The aim of TEM is to examine the ultrastructure of specimens in ways that cannot be examined using other equipments or techniques. In some cases, when the requirement for TEM were made after tissue collection, useful information can be obtained from reprocessing the formalin-fixed, wax-embedded tissue used for light microscopy.
Related Concept Videos
Fixation and Sectioning
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
Preparation of Samples for Electron Microscopy
