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Formulations for Freeze-drying of Bacteria and Their Influence on Cell Survival
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Formulations for Freeze-drying of Bacteria and Their Influence on Cell Survival

Published on: August 3, 2013

Bacteria incorporation in deep-eutectic solvents through freeze-drying

María C Gutiérrez1, María L Ferrer, Luís Yuste

  • 1Instituto de Ciencia de Materiales de Madrid-ICMM, Consejo Superior de Investigaciones Científicas-CSIC, Campus de Cantoblanco, 28049-Madrid, Spain. mcgutierrez@icmm.csic.es

Angewandte Chemie (International Ed. in English)
|November 28, 2009
PubMed
Summary

No abstract available in PubMed .

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Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...

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