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Updated: Jun 18, 2026

Using Caco-2 Cells to Study Lipid Transport by the Intestine
Published on: August 20, 2015
[Transport mechanism of swertiamain metabolite across Caco-2 model]
Cheng Tang1, Yanjun Zhang, Tiejun Zhang
1Pharmacy College, Tianjin Medical University, Tianjin 300070, China.
Objective:
To investigate the intestinal absorption mechanism of swertiamain metabolite {(Z)-5-ethylidene-8-hydroxy-3,4,5,6,7,8-hexahydro-1H-pyrano [3,4-c]pyridine-1-one, EHPO}.
Method:
The time depended hi-directional transport of EHPO in Caco-2 monolayer model was investigated with the factors of concentration, pH and verapamil. EHPO concentration was measured by HPLC assay and the apparent permeability coefficients (Papp) were calculated.
Result:
In the this cell model, EHPO could be absorbed through Caco-2 soon. The P(app AP-BL) was equal to the P(app BL-AP), and Papp keep almost constant with the selected concentration investigated. But the Papp could be influenced by pH and verapamil (100 mg x L(-1)).
Conclusion:
The absorption of EHPO in Caco-2 cell model is a passive one. And absorption of EHPO in the intestines is quite good.
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