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Related Experiment Video

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In Situ Nucleosome Assembly for Single-Molecule Correlative Force and Fluorescence Microscopy

In Situ Nucleosome Assembly for Single-Molecule Correlative Force and Fluorescence Microscopy

Published on: September 6, 2024

Sequence-dependent DNA helical rise and nucleosome stability.

Francesco Pedone¹, Daniele Santoni

¹Dept. of Genetics and Molecular Biology, Sapienza University, P.A. Moro 3, 00161 Rome, Italy. francesco.pedone@uniroma1.it

BMC Molecular Biology

|December 1, 2009

Summary

Nucleosome stability is linked to the symmetric distribution of charges along DNA. Greater symmetry in DNA helical steps correlates with more stable nucleosomes, impacting gene regulation.

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Assembly of Nucleosomal Arrays from Recombinant Core Histones and Nucleosome Positioning DNA

Assembly of Nucleosomal Arrays from Recombinant Core Histones and Nucleosome Positioning DNA

Published on: September 10, 2013

Area of Science:

Molecular Biology
Genetics
Biophysics

Background:

Nucleosomes are fundamental to eukaryotic chromatin structure and gene expression regulation.
The nucleosome particle exhibits a symmetric distribution of positive charges on its histone surface, interacting with DNA's negative phosphate backbone.

Purpose of the Study:

To investigate the relationship between DNA sequence symmetry and nucleosome stability.
To identify DNA sequence features that dictate nucleosome positioning and stability.

Main Methods:

Analysis of helical DNA steps in known nucleosomal sequences.
Comparison of distances around the nucleosomal dyad axis in synthetic DNA sequences.
Evaluation of symmetry patterns in DNA sequences from promoters and known nucleosome positions.

Main Results:

A significant correlation exists between the symmetric distribution of helical steps and nucleosome stability.
Increased symmetry in DNA distances around the dyad axis leads to more stable nucleosomes.
Palinstases, identified by symmetric distribution curves, indicate preferential nucleosome dyad positioning sites.

Conclusions:

Nucleosome stability is directly influenced by the symmetry of DNA sequences.
Palinstases provide insights into preferred nucleosome positioning.
Nucleosomes play a significant role in transcription termination, showing high affinity at termination sites.