Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Mass Spectrometry: Complex Analysis

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...

Capillary Electrophoresis: Instrumentation

Capillary Electrophoresis: Instrumentation

Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...

Electrospray Ionization (ESI) Mass Spectrometry

Electrospray Ionization (ESI) Mass Spectrometry

Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...

Capillary Electrophoresis: Applications

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...

Electrophoresis: Overview

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...

High-Performance Liquid Chromatography: Instrumentation

High-Performance Liquid Chromatography: Instrumentation

High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Estimating the solubility of organic compounds in water using electrokinetic chromatography.

Journal of chromatography. A·2026

Same author

Separation and Analysis of Chiral Compounds by Nano-Liquid Chromatography Using Glycopeptide Antibiotics.

Methods in molecular biology (Clifton, N.J.)·2025

Same author

Chiral Separations by Using Capillary Electrochromatography: An Overview.

Methods in molecular biology (Clifton, N.J.)·2025

Same author

Natural Eutectic Solvent-Based Temperature-Controlled Liquid-Liquid Microextraction and Nano-Liquid Chromatography for the Analysis of Herbal Aqueous Samples.

Foods (Basel, Switzerland)·2025

Same author

Recovery of cellulose acetate bioplastic from cigarette butts: realization of a sustainable sorbent for water remediation.

The Science of the total environment·2024

Same author

A liquid chromatography-mass spectrometry method for the enantioselective multiresidue determination of nine chiral agrochemicals in urine using an enrichment procedure based on graphitized carbon black.

Analytical and bioanalytical chemistry·2023

Search research articles

Related Experiment Video

Updated: Jun 18, 2026

Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples

Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples

Published on: October 1, 2016

Coupling capillary electrochromatography with mass spectrometry by using a liquid-junction nano-spray interface.

Giovanni D'Orazio¹, Salvatore Fanali

¹Institute of Chemical Methodologies, National Research Council, Area della Ricerca di Roma I, Via Salaria Km 29.300, 00015 Monterotondo Scalo (Rome), Italy.

Journal of Chromatography. A

|December 1, 2009

Summary

Capillary electrochromatography coupled with mass spectrometry (CEC-MS) effectively separates pesticides and drug enantiomers. Optimization of hydrostatic pressure enhanced ion signals for sensitive detection.

More Related Videos

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry

Published on: October 24, 2018

Capillary Electrophoresis Mass Spectrometry Approaches for Characterization of the Protein and Metabolite Corona Acquired by Nanomaterials

Capillary Electrophoresis Mass Spectrometry Approaches for Characterization of the Protein and Metabolite Corona Acquired by Nanomaterials

Published on: October 27, 2020

Related Experiment Videos

Last Updated: Jun 18, 2026

Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples

Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples

Published on: October 1, 2016

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry

Published on: October 24, 2018

Capillary Electrophoresis Mass Spectrometry Approaches for Characterization of the Protein and Metabolite Corona Acquired by Nanomaterials

Capillary Electrophoresis Mass Spectrometry Approaches for Characterization of the Protein and Metabolite Corona Acquired by Nanomaterials

Published on: October 27, 2020

Area of Science:

Analytical Chemistry
Separation Science

Background:

Capillary electrochromatography (CEC) offers high-efficiency separations.
Coupling CEC with mass spectrometry (MS) enhances detection capabilities.
Challenges exist in interfacing CEC with MS, particularly regarding flow and ionization.

Purpose of the Study:

To develop and optimize a capillary electrochromatography-mass spectrometry (CEC-MS) method.
To evaluate the separation of pesticides and pharmaceutical enantiomers using CEC-MS.
To investigate the impact of interface hydrostatic pressure on MS signal intensity.

Main Methods:

CEC separations were performed in fused silica capillaries packed with RP(18) silica or vancomycin-modified silica.
A laboratory-made liquid-junction interface connected the CEC capillary to the MS.
Hydrostatic pressure was applied at the interface to optimize ion spray and MS detection.

Main Results:

Optimized hydrostatic pressure at the interface increased MS ion signal linearly up to 3.5 kPa.
Pesticide separations were unaffected by interface pressure adjustments.
Good resolution was achieved for basic enantiomeric compounds, with limits of detection between 0.24-0.60 µg/mL.

Conclusions:

The developed CEC-MS method is suitable for separating and detecting pesticides and drug enantiomers.
Interface hydrostatic pressure is a critical parameter for optimizing MS sensitivity in CEC-MS.
The technique demonstrates potential for analyzing chiral pharmaceuticals.