Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A novel HIV-1 isolate containing alterations affecting the NF-kappa B element.

G Englund1, M D Hoggan, T S Theodore

  • 1Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892.

Virology
|March 1, 1991
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Co-composting Rugulopteryx okamurae, Alperujo and local organic waste: a sustainable strategy for odour control and compost quality improvement.

Waste management (New York, N.Y.)·2026
Same author

HS-GC determination of atmospheric greenhouse gases with direct in-vial sampling and multiple headspace extraction as an in-vial dilution strategy.

Analytica chimica acta·2026
Same author

Decoding drought tolerance from a genomic approach in Castanea sativa Mill.

The plant genome·2025
Same author

Odour impact simulation of a large urban wastewater treatment plant through the numerical solution of a Eulerian model.

Journal of environmental management·2024
Same author

Assessment of nitrification process in a sequencing batch reactor: Modelling and genomic approach.

Environmental research·2024
Same author

Integral evaluation of effective conversion of sewage sludge from WWTP into highly porous activated carbon.

Journal of environmental management·2023

Distinct HIV-1 clones showed varied replication due to changes in the viral LTR enhancer. These alterations, involving NF-kappa B motifs, impacted promoter activity and viral capacity.

Area of Science:

  • Virology
  • Molecular Biology
  • Immunology

Background:

  • Human Immunodeficiency Virus type 1 (HIV-1) exhibits genetic diversity.
  • Understanding the molecular basis of HIV-1 replication and pathogenesis is crucial for therapeutic development.

Purpose of the Study:

  • To investigate the molecular determinants of distinct replicative and cytopathic properties among HIV-1 clones.
  • To elucidate the role of the viral Long Terminal Repeat (LTR) enhancer region in HIV-1 phenotypic variation.

Main Methods:

  • Propagation of HIV-1 molecular clones in human T cell lines.
  • Nucleotide sequencing and Polymerase Chain Reaction (PCR) analysis to identify genetic alterations.
  • Construction and analysis of LTR-driven CAT (chloramphenicol acetyltransferase) expression vectors.

Related Experiment Videos

Main Results:

  • Three HIV-1 clones from a single isolate (AL1) displayed significant differences in replication and cytopathic effects.
  • Phenotypic variations were largely attributed to modifications within the viral LTR, specifically involving duplications or deletions of the NF-kappa B motif.
  • These LTR alterations were present in the original AL1 virus stock.
  • Subcloning experiments confirmed a direct correlation between NF-kappa B enhancer activity and viral replicative/cytopathic capacity.

Conclusions:

  • The NF-kappa B motif within the HIV-1 LTR enhancer is a critical determinant of viral replication and cytopathic potential.
  • Genetic variations in enhancer elements can lead to distinct phenotypic properties within HIV-1 populations.
  • These findings highlight the importance of enhancer region plasticity in HIV-1 evolution and pathogenesis.