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Inhibitors of Virion Maturation and Assembly01:19

Inhibitors of Virion Maturation and Assembly

As part of their replication cycle, certain viruses synthesize long precursor proteins called polyproteins within infected host cells. In human immunodeficiency virus (HIV), two major polyproteins are produced: Gag and Gag-Pol. The Gag polyprotein supplies the structural components of the virus, while Gag-Pol includes essential viral enzymes such as reverse transcriptase, integrase, and protease. After synthesis, these polyproteins move to the host cell membrane, where they assemble into an...
Protein Complex Assembly02:41

Protein Complex Assembly

Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
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Protein-protein Interfaces02:04

Protein-protein Interfaces

Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a polypeptide...
Protein-Protein Interfaces02:04

Protein-Protein Interfaces

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Conjugated Proteins02:50

Conjugated Proteins

Simple proteins and protein complexes contain only amino acids. In contrast, many other proteins, called conjugated proteins, covalently bond with non-protein moieties.
Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...
Inhibitors Of Virion Release01:25

Inhibitors Of Virion Release

Viral replication and dissemination rely on efficient mechanisms for host cell entry, genome replication, assembly, and release. Influenza viruses, such as types A and B, are negative-sense single-stranded RNA viruses with a segmented genome, that depend on two critical surface glycoproteins to carry out these processes: hemagglutinin (HA) and neuraminidase (NA). HA initiates infection by binding to sialic acid residues on the surface of host epithelial cells, facilitating receptor-mediated...

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Updated: Jun 18, 2026

A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions
13:56

A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions

Published on: July 18, 2013

Poxvirus proteomics and virus-host protein interactions.

Kim Van Vliet1, Mohamed R Mohamed, Leiliang Zhang

  • 1Department of Molecular Genetics and Microbiology, University of Florida College of Medicine, 1600 SW Archer Road, Box 100266, Gainesville, FL 32610, USA.

Microbiology and Molecular Biology Reviews : MMBR
|December 1, 2009
PubMed
Summary

Poxvirus proteomics reveals viral protein functions and interactions. These studies enhance understanding of viral pathogenesis and aid in drug discovery by identifying viral and host binding partners.

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Last Updated: Jun 18, 2026

A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions
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Published on: July 18, 2013

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Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins
08:26

Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins

Published on: August 31, 2017

Area of Science:

  • Virology
  • Proteomics
  • Molecular Biology

Background:

  • Poxvirus genomes encode functional proteins crucial for viral composition and interactions.
  • Understanding virus-virus and virus-host protein interactions offers insights into viral pathogenesis and drug discovery.
  • Traditional methods like yeast two-hybrid and coimmunoprecipitation are used to study protein-protein interactions.

Purpose of the Study:

  • To review the application of proteomic methodologies in studying poxvirus proteins.
  • To highlight the identification of viral components and virus-host protein interactions.
  • To discuss advanced techniques for characterizing viral protein functions and interactions.

Main Methods:

  • Proteomic techniques including mass spectrometry for identifying viral protein complexes.
  • Yeast two-hybrid studies and coimmunoprecipitations for characterizing protein-protein interactions.
  • Structural studies to elucidate viral protein functions and virus-host interactions.

Main Results:

  • Proteomics enables the identification of viral protein components within larger complexes.
  • Mass spectrometry and structural studies provide new information on viral protein functions.
  • Identifying binding partners (viral or host) helps determine the putative functions of unknown viral proteins.

Conclusions:

  • Poxvirus proteomics is a powerful approach for dissecting viral composition and function.
  • Proteomic methodologies are essential for understanding virus-host interactions and pathogenesis.
  • Advanced proteomic techniques and validation methods are crucial for future poxvirus research and drug development.