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Updated: Jun 18, 2026
Generation of High Quality Chromatin Immunoprecipitation DNA Template for High-throughput Sequencing (ChIP-seq)
Published on: April 19, 2013
Chromatin profiling by directly sequencing small quantities of immunoprecipitated DNA
Alon Goren1, Fatih Ozsolak, Noam Shoresh
1Broad Institute of Harvard and Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
None:
Chromatin structure and transcription factor localization can be assayed genome-wide by sequencing genomic DNA fractionated by protein occupancy or other properties, but current technologies involve multiple steps that introduce bias and inefficiency. Here we apply a single-molecule approach to directly sequence chromatin immunoprecipitated DNA with minimal sample manipulation. This method is compatible with just 50 pg of DNA and should thus facilitate charting chromatin maps from limited cell populations.
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02:36Chromatin Immunoprecipitation- ChIP
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