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Related Concept Videos

Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
Loss of Tumor Suppressor Gene Functions01:12

Loss of Tumor Suppressor Gene Functions

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
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Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
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The Intrinsic Apoptotic Pathway01:31

The Intrinsic Apoptotic Pathway

Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...

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Related Experiment Video

Updated: Jun 18, 2026

Purification of Ubiquitinated p53 Proteins from Mammalian Cells
10:55

Purification of Ubiquitinated p53 Proteins from Mammalian Cells

Published on: March 21, 2022

Inactivation of HAUSP in vivo modulates p53 function.

N Kon1, Y Kobayashi, M Li

  • 1Institute for Cancer Genetics, Columbia University, New York, NY 10032, USA.

Oncogene
|December 1, 2009
PubMed
Summary
This summary is machine-generated.

The deubiquitinase Hausp is essential for embryonic development, regulating the p53-Mdm2 pathway. Its absence causes embryonic lethality due to reduced proliferation, highlighting Hausp

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Yeast As a Chassis for Developing Functional Assays to Study Human P53
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Yeast As a Chassis for Developing Functional Assays to Study Human P53
14:57

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

Area of Science:

  • Molecular Biology
  • Developmental Biology
  • Biochemistry

Background:

  • Hausp (Hepatoma Upstream Protein) is a deubiquitinase enzyme.
  • Hausp regulates the p53-Mdm2 pathway, influencing p53 stability and activity.
  • The precise physiological role of Hausp in embryonic development is not fully understood.

Purpose of the Study:

  • To investigate the physiological functions of Hausp in vivo.
  • To determine the role of Hausp in embryonic development and the p53-Mdm2 pathway.

Main Methods:

  • Generation of hausp knockout mice.
  • Analysis of embryonic development, p53 activation, apoptosis, and proliferation in knockout embryos.
  • Generation and analysis of hausp and p53 double knockout embryos.

Main Results:

  • Hausp knockout mice exhibit embryonic lethality between E6.5 and E7.5.
  • Hausp knockout embryos show p53 activation but no significant increase in apoptosis.
  • Embryonic lethality is linked to reduced proliferation and developmental termination, involving both p53-dependent and -independent functions.

Conclusions:

  • Hausp plays a critical role in embryonic development.
  • Hausp is essential for regulating the p53-Mdm2 pathway during embryogenesis.
  • The absence of Hausp leads to developmental defects primarily through reduced cell proliferation.