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Updated: Jun 18, 2026
10:55
Purification of Ubiquitinated p53 Proteins from Mammalian Cells
Published on: March 21, 2022
Inactivation of HAUSP in vivo modulates p53 function
N Kon1, Y Kobayashi, M Li
1Institute for Cancer Genetics, Columbia University, New York, NY 10032, USA.
Oncogene
|December 1, 2009
Summary
The deubiquitinase Hausp is essential for embryonic development, regulating the p53-Mdm2 pathway. Its absence causes embryonic lethality due to reduced proliferation, highlighting Hausp
Area of Science:
- Molecular Biology
- Developmental Biology
- Biochemistry
Background:
- Hausp (Hepatoma Upstream Protein) is a deubiquitinase enzyme.
- Hausp regulates the p53-Mdm2 pathway, influencing p53 stability and activity.
- The precise physiological role of Hausp in embryonic development is not fully understood.
Purpose of the Study:
- To investigate the physiological functions of Hausp in vivo.
- To determine the role of Hausp in embryonic development and the p53-Mdm2 pathway.
Main Methods:
- Generation of hausp knockout mice.
- Analysis of embryonic development, p53 activation, apoptosis, and proliferation in knockout embryos.
- Generation and analysis of hausp and p53 double knockout embryos.
Main Results:
- Hausp knockout mice exhibit embryonic lethality between E6.5 and E7.5.
- Hausp knockout embryos show p53 activation but no significant increase in apoptosis.
- Embryonic lethality is linked to reduced proliferation and developmental termination, involving both p53-dependent and -independent functions.
Conclusions:
- Hausp plays a critical role in embryonic development.
- Hausp is essential for regulating the p53-Mdm2 pathway during embryogenesis.
- The absence of Hausp leads to developmental defects primarily through reduced cell proliferation.
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