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Updated: Jun 18, 2026

Microplot Design and Plant and Soil Sample Preparation for 15Nitrogen Analysis
Published on: May 10, 2020
[Effects of water-nitrogen interaction on soil water utilization by wheat and fructan content in wheat stem]
Xiao-Yan Wang1, Cheng-Yan Zheng, Zhen-Wen Yu
1College of Agronomy, Yangtze University, Jingzhou, Hubei, China. wamail_wang@163.com
Abstract:
A field experiment was conducted to study the effects of water-nitrogen interaction on the flag leaf photosynthetic rate, penult stem fructan content, fertilizer N use efficiency, and soil water use efficiency of strong gluten wheat variety 'Jimai 20'. Three N application levels (N0, 0 kg x hm(-2); N1, 180 kg x hm(-2); and N2, 240 kg x hm(-2)) and four irrigation schedules (W0, no irrigation; W1, irrigation before sowing and at jointing and flowering stages; W2, irrigation before sowing, before wintering, and at jointing and flowering stages; W3, irrigation before sowing, before wintering, and at jointing, flowering, and grain-filling stages) were designed. The irrigation amount was 60 mm each time. Among the N treatments, treatment N1 had a higher flag leaf net photosynthetic rate and penult stem fructan content, and the highest grain yield, apparent N use efficiency, agronomic N use efficiency, and water use efficiency. Treatment N2 had a higher penult stem fructan content than treatments N0 and N1. No N application and applying excessive N did not benefit the increase of grain yield, fertilizer N use efficiency, and water use efficiency. Treatment W1 promoted the fructan accumulation in penult stem and accelerated the translocation of the accumulated fructan to grain, being beneficial to the increase of grain yield. Treatment N1W1 had the highest grain yield, and higher fertilizer N use efficiency and water use efficiency. Excessive irrigation and N application increased the flag leaf net photosynthetic rate and penult stem fructan content, but decreased the fertilizer N use efficiency and water use efficiency, with no significant effects on grain yield.
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