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Related Experiment Video

Updated: Jun 18, 2026

Medium-throughput Screening Assays for Assessment of Effects on Ca2+-Signaling and Acrosome Reaction in Human Sperm

Medium-throughput Screening Assays for Assessment of Effects on Ca²⁺-Signaling and Acrosome Reaction in Human Sperm

Published on: March 1, 2019

[Recombinant human testis sperm binding protein increases sperm motility parameters].

Wen-hui Su¹, Xin-chun Liu, Chen Feng

¹Department of Biochemistry and Molecular Biology, School of Basic Medicine, China Medical University, Shenyang, Liaoning 110001, China. suwenhui616@hotmail.com

Zhonghua Nan Ke Xue = National Journal of Andrology

|December 2, 2009

Summary

Recombinant human testis sperm binding protein (TSBP) at 0.1 mg/ml improved sperm motility in healthy men and increased forward motility in asthenospermia patients in vitro. This protein shows potential for enhancing sperm function.

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Last Updated: Jun 18, 2026

Medium-throughput Screening Assays for Assessment of Effects on Ca2+-Signaling and Acrosome Reaction in Human Sperm

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Sperm Collection of Differential Quality Using Density Gradient Centrifugation

Sperm Collection of Differential Quality Using Density Gradient Centrifugation

Published on: November 29, 2018

Area of Science:

Reproductive Biology
Molecular Biology
Biochemistry

Context:

Sperm motility is crucial for male fertility.
Asthenospermia, characterized by poor sperm motility, is a common cause of male infertility.
Investigating novel therapeutic targets for male infertility is essential.

Purpose:

To evaluate the in vitro effects of recombinant human testis sperm binding protein (TSBP) on human sperm motility parameters.
To determine the optimal concentration and incubation time for TSBP's effect on sperm function.

Summary:

Recombinant His6-TSBP at 0.1 mg/ml significantly increased forward sperm motility in healthy fertile men after 1 hour and both motility and forward motility after 3 hours.
In asthenospermia patients, the same concentration of His6-TSBP increased the forward motile sperm percentage after 3 hours.
Lower concentrations (0.01 mg/ml) of His6-TSBP showed no significant effect on sperm motility parameters.

Impact:

Recombinant His6-TSBP demonstrates potential as a therapeutic agent to improve sperm motility in infertile men.
This finding could lead to new strategies for treating male factor infertility.
Further research may elucidate the precise mechanism of TSBP action on sperm.