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Molecular detection of viable bacterial pathogens in water by ratiometric pre-rRNA analysis

Gerard A Cangelosi1, Kris M Weigel, Clarita Lefthand-Begay

  • 1Seattle Biomedical Research Institute, Seattle, Washington 98109, USA.

Applied and Environmental Microbiology
|December 2, 2009
PubMed

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...