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Defining Substrate Specificities for Lipase and Phospholipase Candidates
Published on: November 23, 2016
Enhanced display of lipase on the Escherichia coli cell surface, based on transcriptome analysis
Jong Hwan Baek1, Mee-Jung Han, Seung Hwan Lee
1Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Program), BioProcess Engineering Research Center, Dongyang University, Yeongju, Gyeongbuk 750-711, Republic of Korea.
Applied and Environmental Microbiology
|December 2, 2009
Abstract:
A cell surface display system was developed using Escherichia coli OmpC as an anchoring motif. The fused Pseudomonas fluorescens SIK W1 lipase was successfully displayed on the surface of E. coli cells, and the lipase activity could be enhanced by the coexpression of the gadBC genes identified by transcriptome analysis.
