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DNA Isolation

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

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Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes

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Published on: July 17, 2021

DNA from buccal swabs suitable for high-throughput SNP multiplex analysis.

Gai L McMichael¹, Catherine S Gibson, Michael E O'Callaghan

¹The University of Adelaide, Disciplines of Obstetrics and Gynaecology, Women's and Children's Hospital, Adelaide, Australia. gai.mcmichael@adelaide.edu.au

Journal of Biomolecular Techniques : JBT

|December 2, 2009

Summary

Noninvasive buccal swabs and a commercial DNA extraction kit offer a convenient, cost-effective method for genetic material collection. This approach yields sufficient DNA quantity and quality for high-throughput single nucleotide polymorphism (SNP) analysis.

Keywords:

single nucleotide polymorphism

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Published on: August 27, 2014

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Area of Science:

Genetics
Molecular Biology
Biotechnology

Background:

Collecting genetic material for large-scale studies requires methods that are noninvasive, cost-effective, and suitable for self-collection and mailing.
High-throughput single nucleotide polymorphism (SNP) analysis demands DNA of specific quantity and quality.

Purpose of the Study:

To identify a convenient, reliable, and inexpensive noninvasive method for genetic material collection.
To find a compatible commercial DNA extraction protocol for high-throughput SNP multiplex analysis.

Main Methods:

A pilot study evaluated commercially available buccal swabs and DNA extraction kits using 34 individuals.
DNA yield was quantified using Picogreen dsDNA, and integrity was assessed via SNP multiplex assays.
Selected methods were applied to a large cohort of 1140 samples.

Main Results:

The Catch-All swabs and Isohelix DNA isolation kit were chosen based on pilot study performance.
Average DNA yield in the pilot study (n=34) was 1.94 µg ± 0.54, with a 94% genotyping pass rate.
In the high-throughput application (n=1140), average DNA yield was 2.44 µg ± 1.74, with a ≥93% genotyping pass rate.

Conclusions:

Catch-All buccal swabs provide a convenient and cost-effective alternative to blood sampling for genetic material collection.
The combination of Catch-All swabs and Isohelix DNA isolation kit delivers adequate DNA quantity and quality for high-throughput SNP multiplex analysis.