Sex matters in the establishment of murine tendon composition and material properties during growth

The existence of sex-based differences in tendon and ligament injury rates has led investigators to test the hypothesis that sex plays a significant role in modulating tendon and ligament composition and material properties. To date, no studies have attempted to characterize how such differences develop during the course of normal tissue maturation and growth. Thus, the primary aim of the present study was to use a murine model to test the hypothesis that sex-based differences in the normal age-related development of tendon composition and material properties exist by assessing these parameters in the Achilles and tail tendons from 4-, 6-, 9-, 12-, and 15-week-old male and female C57Bl/6J mice. Despite significantly lower levels of total collagen content in females subsequent to sexual maturity (p<0.0001), as well as a significant effect of sex on glycosaminoglycan content (p<0.0001), Achilles tendon elastic modulus was not compromised in females. Female Achilles tendons did exhibit a significantly higher failure strain (p=0.0201) and strain energy density (p=0.0004) than did males, as well as a trend toward higher ultimate strength (p=0.0556). In contrast to the high load-bearing environment of the Achilles tendon site, sex did not have a statistically significant effect on any compositional or material property in the low load-bearing tendon fascicles of the tail. These data support recent studies by others, which suggest that male and female tendons have a differential adaptational response to their local mechanical loading environment.