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Updated: Jun 18, 2026

Separation of Single-stranded DNA, Double-stranded DNA and RNA from an Environmental Viral Community Using Hydroxyapatite Chromatography
Published on: September 29, 2011
Relationship between human IgG structure and retention time in hydroxyapatite chromatography with sodium-phosphate
Taishiro Nakagawa1, Takashi Ishihara, Hideaki Yoshida
1Bio Process Research and Development Laboratories, Production Division, Kyowa Hakko Kirin, Co., Ltd., Gunma, Japan. taishiro.nakagawa@kyowa-kirin.co.jp
Abstract:
The aim of this study was to classify the retention time of recombinant human monoclonal antibodies (rhMAbs) in hydroxyapatite chromatography with sodium-phosphate gradient elution according to their physicochemical properties. We analyzed 37 rhMAbs and found that (i) the structures of both the constant and variable regions affected retention time, (ii) the number of basic amino acid residues in the variable region, particularly in the heavy chain, correlated well with retention time, and (iii) this correlation was more pronounced (e.g. r(2)=0.87 for 18 kappa IgG(1) rhMAbs) when the surface accessibility of those residues are taken into account. These findings provide a useful guide for investigators and purification-process developers working with monoclonal antibodies.
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