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Cell Surface Marker Mediated Purification of iPS Cell Intermediates from a Reprogrammable Mouse Model

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Published on: September 6, 2014

Preview. A shore sign of reprogramming.

Steven M Pollard¹, Stefan H Stricker, Stephan Beck

¹Wellcome Trust Centre for Stem Cell Biology and Department of Biochemistry, University of Cambridge, Cambridge CB2 1QR, UK; UCL Cancer Institute, University College London, London WC1E 6BT, UK.

|December 3, 2009

Summary

CpG island shores in mammalian cells exhibit differentially methylated regions (DMRs). These DMRs are reconfigured during induced cellular reprogramming, as shown by recent studies.

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Area of Science:

Epigenetics
Genomics
Cellular Biology

Background:

CpG methylation patterns are crucial for mammalian genome regulation.
Differentially methylated regions (DMRs) are frequently found at CpG island shores.

Purpose of the Study:

To investigate the dynamic changes in CpG methylation patterns during induced reprogramming.
To determine if DMRs at CpG island shores are altered during cellular reprogramming.

Main Methods:

Genome-wide analysis of CpG methylation patterns.
Comparison of methylation profiles before and after induced reprogramming.

Main Results:

Identification of numerous DMRs located at CpG island shores.
Demonstration of significant reconfiguration of these DMRs during induced reprogramming.

Conclusions:

CpG island shore methylation is dynamic and undergoes substantial changes during cellular reprogramming.
These findings provide insights into the epigenetic mechanisms underlying induced pluripotency.