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Updated: Jun 18, 2026

Super-Resolution Imaging of Bacterial Secreted Proteins Using Genetic Code Expansion
Published on: February 10, 2023
Signal sequence is still required in genes downstream of "autocleaving" 2A peptide for secretary or membrane-anchored
Jun Yan1, Hongjun Wang, Qian Xu
1Department 4, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing 400042, People's Republic of China.
Abstract:
2A Peptide sequences are now being widely used to construct multicistronic expression vectors. It is suggested that when only the first 2A-linked protein bears a signal sequence, the signal-less protein(s) downstream of 2A can also be translocated into the mammalian endoplasmic reticulum system through a "slipstreaming" mechanism. By using flow cytometry and cell surface CD90 as a localization indicator, we show here that slipstreaming translocation does not occur in mammalian cells; that is, the second protein downstream of 2A still requires signal sequence for secretary or membrane-anchored expression.
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