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Updated: Jun 18, 2026
Physiology Lab Demonstration: Glomerular Filtration Rate in a Rat
Published on: July 26, 2015
Evaluation of a single sampling method for estimation of plasma iohexol clearance in dogs and cats with various
Yuichi Miyagawa1, Naoyuki Takemura, Hisashi Hirose
1Laboratory of Veterinary Integrative Medicine, School of Veterinary Medicine, Faculty of Veterinary Science, Nippon Veterinary and Life Science University, 1-7-1 Kyonan Cho, Musashino City, Tokyo, 180-8602, Japan. d0607@nvlu.ac.jp
Abstract:
Plasma iohexol clearance (PCio) is a practical method for measuring the glomerular filtration rate (GFR) in clinical settings. However, it is too time-consuming for routine application and requires hospitalization for at least half a day. Therefore, the development of a simpler procedure for plasma clearance is necessary to allow the frequent measurement of GFR in clinical settings. The purpose of the present study was to evaluate a single sampling method for estimation of PCio in dogs and cats with various kidney functions. The PCio determined by the 1-compartment model using 3 samples (PCio (3samples)) was used as a reference for the evaluation of the single sampling method (PCio (single)). Plasma iohexol concentration was determined by a cerium arsenite colorimetric method. PCio single was calculated using the equation obtained by nonlinear regression analysis. PCio (single) was significantly correlated with PCio (3samples) in both dogs and cats (dogs: R(2)=0.985, P<0.001, cats: R(2)=0.986, P<0.001). In a receiver operating characteristics analysis, the area under the curve, sensitivity, and specificity for detecting decreased GFR were 0.995 [SE, 0.003], 98%, and 93% for dogs and 0.993 [SE, 0.003], 98%, and 93% for cats, respectively. These results demonstrate that PCio (single) may be very useful for the detection of decreasing GFR in dogs and cats.
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