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Proteome of Salmonella Enterica Serotype Typhimurium Grown in a Low Mg/pH Medium

Liang Shi1, Charles Ansong, Heather Smallwood

  • 1Pacific Northwest National Laboratory, Richland, Washington 99352.

Journal of Proteomics & Bioinformatics
|December 3, 2009
PubMed

Insights

Low magnesium and pH conditions alter Salmonella Typhimurium proteome, affecting type III secretion system (T3SS) and biotin synthesis. Biotin synthesis is crucial for bacterial replication during macrophage infection.

Area of Science:

  • Microbiology
  • Proteomics
  • Bacterial Pathogenesis

Background:

  • Salmonella enterica serotype Typhimurium is a significant foodborne pathogen.
  • Understanding bacterial adaptation to environmental stress is crucial for controlling infections.

Purpose of the Study:

  • To investigate the proteomic changes in Salmonella Typhimurium cultured in a low magnesium/pH medium (MgM).
  • To determine the impact of MgM Shock and MgM Dilution conditions on Salmonella Typhimurium.
  • To assess the role of specific protein changes in bacterial virulence and survival.

Main Methods:

  • Global proteomic analysis of Salmonella Typhimurium.
  • Culturing bacteria under MgM Shock and MgM Dilution conditions.
  • Western blot analysis and gene deletion studies in RAW 264.7 macrophages.

Main Results:

  • MgM Shock and MgM Dilution differentially modulated the Salmonella Typhimurium proteome.
  • MgM Shock induced proteins associated with low oxygen levels.
  • MgM Dilution induced type III secretion system (SPI2-T3SS) and biotin biosynthesis proteins.
  • Biotin biosynthesis protein BioB is essential for Salmonella Typhimurium replication within macrophages.

Conclusions:

  • Salmonella Typhimurium adapts to low Mg(2+)/pH by altering its proteome, impacting virulence factors and metabolic pathways.
  • The SPI2-T3SS and biotin biosynthesis are upregulated under specific MgM conditions.
  • Biotin availability is a limiting factor for Salmonella Typhimurium during macrophage infection, highlighting a potential therapeutic target.

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