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First identification of Neospora caninum by PCR in aborted bovine foetuses in Romania

Ovidiu Suteu1, Adriana Titilincu, David Modrý

  • 1Department of Parasitology and Parasitic Diseases, University of Agricultural Science and Veterinary Medicine, Faculty of Veterinary Medicine, 3-5 Mănăştur Street, Cluj Napoca, Romania.

Parasitology Research
|December 3, 2009
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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...