Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Gap junctional communication during limb cartilage differentiation.

C N Coelho1, R A Kosher

  • 1Department of Anatomy, University of Connecticut Health Center, Farmington 06032.

Developmental Biology
|March 1, 1991
PubMed
Summary

Cell-cell communication via gap junctions is crucial for cartilage differentiation during embryonic limb development. This study highlights their role in chondrogenesis, particularly in differentiating cartilage cells.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

FGFR2 signaling in normal and limbless chick limb buds.

Developmental genetics·1999
Same author

Dlx-5 in limb initiation in the chick embryo.

Developmental dynamics : an official publication of the American Association of Anatomists·1999
Same author

Syndecan-3 in limb skeletal development.

Microscopy research and technique·1998
Same author

Ectopic expression of Msx-2 in posterior limb bud mesoderm impairs limb morphogenesis while inducing BMP-4 expression, inhibiting cell proliferation, and promoting apoptosis.

Developmental biology·1998
Same author

FGF-stimulated outgrowth and proliferation of limb mesoderm is dependent on syndecan-3.

Developmental biology·1997
Same author

Syndecan-3 and the control of chondrocyte proliferation during endochondral ossification.

Experimental cell research·1996

Area of Science:

  • Developmental Biology
  • Cell Biology
  • Biochemistry

Background:

  • Limb bud development involves mesenchymal cell condensation preceding cartilage formation.
  • Cell-cell interactions are essential for triggering chondrogenic differentiation.
  • Gap junctions facilitate direct intercellular communication.

Purpose of the Study:

  • To investigate the role of gap junction communication in chondrogenesis during embryonic limb development.
  • To determine if gap junction activity is specific to differentiating cartilage cells.

Main Methods:

  • Utilized high-density micromass cultures of embryonic chick wing bud cells.
  • Assayed gap junction communication using intercellular transfer of lucifer yellow dye.
  • Compared communication in homogeneous and heterogeneous cell populations.

Related Experiment Videos

Main Results:

  • Extensive gap junction communication was observed during cell condensation and chondrogenesis in homogeneous cultures.
  • In heterogeneous cultures, significant gap junction activity was limited to differentiating cartilage cells.
  • Non-chondrogenic connective tissue lineage cells showed minimal gap junction communication.

Conclusions:

  • Gap junction-mediated cell-cell communication plays a significant role in regulating limb cartilage differentiation.
  • This communication appears specific to the chondrogenic lineage during limb development.
  • Further research is warranted to fully elucidate the mechanisms involved.