Related Experiment Video

Updated: Oct 8, 2026

RhoC GTPase Activation Assay
09:58

RhoC GTPase Activation Assay

Published on: August 22, 2010

Characterization of RhoA-mediated chemoresistance in gastric cancer cells

Won Ki Kang1, Inkyoung Lee, Chaehwa Park

  • 1Cancer Center, Samsung Medical Center, and Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Seoul, Korea.

Cancer Research and Treatment
|December 4, 2009
PubMed
Abstract

Insights

High RhoA expression in gastric cancer cells increases resistance to certain chemotherapeutic drugs like taxol and vincristine. Targeting RhoA may improve treatment effectiveness.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Oncology

Background:

  • RhoA is a key signaling molecule regulating cell behavior.
  • Its role in cancer drug resistance is not fully understood.

Purpose of the Study:

  • To investigate the influence of RhoA on gastric cancer cell sensitivity to chemotherapy.
  • To explore potential therapeutic strategies targeting RhoA.

Main Methods:

  • Gastric cancer cells (SNU638) were transfected to alter RhoA levels.
  • MTT assays and Western blot analysis assessed drug response and cell death.
  • cDNA microarray analysis identified differentially expressed genes.

Main Results:

  • RhoA overexpression conferred resistance to lovastatin, 5-FU, taxol, and vincristine.
  • RhoA reduced Poly (ADP-ribose) polymerase (PARP) cleavage and cell death.
  • Gene expression changes indicated RhoA involvement in anti-oxidant defense, metabolism, and anti-apoptosis.

Conclusions:

  • Elevated RhoA expression in gastric cancer cells correlates with resistance to specific chemotherapeutics.
  • Inhibiting RhoA may enhance the efficacy of drugs like taxol and vincristine.
  • RhoA modulation presents a potential therapeutic avenue for gastric cancer treatment.
Keywords:
Microarray analysisResistanceRho

More Related Videos

Looking for Driver Pathways of Acquired Resistance to Targeted Therapy: Drug Resistant Subclone Generation and Sensitivity Restoring by Gene Knock-down08:59

Looking for Driver Pathways of Acquired Resistance to Targeted Therapy: Drug Resistant Subclone Generation and Sensitivity Restoring by Gene Knock-down

Published on: December 11, 2017

Multi-Gene Single Nucleotide Polymorphism Detection in Gastric Cancer Based on Ion Semiconductor Sequencing Platform06:21

Multi-Gene Single Nucleotide Polymorphism Detection in Gastric Cancer Based on Ion Semiconductor Sequencing Platform

Published on: May 10, 2024

Related Concept Videos

Adaptive Mechanisms in Cancer Cells02:53

Adaptive Mechanisms in Cancer Cells

Cancer cells accumulate genetic changes at an abnormally rapid rate due to the defects in the DNA repair mechanisms. From an evolutionary perspective, such genetic instability is advantageous for cancer development. Mutant cell lines accumulate a series of beneficial mutations that contribute to their progression into cancer.
Some of the advantages that cancer cells have on normal cells include - enhanced ability to divide without terminally differentiating, induce new blood vessel formation,...
6.8K
The Ras Gene02:38

The Ras Gene

The Ras-gene-encoded proteins are regulators of signaling pathways controlling cell proliferation, differentiation, or cell survival. The Ras-gene family in humans constitutes three primary members—the HRas, NRas, and KRas. These genes code for four functionally distinct yet closely related proteins—the HRas, NRas, KRas4A, and KRas4B. The involvement of mutant Ras genes in human cancer was first discovered in 1982 and is among the most common causes of human tumorigenesis.
Ras is a...
6.9K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
4.5K
Treatment Resistant Cancers02:56

Treatment Resistant Cancers

Cancer is the second leading cause of death in the United States. A cancer cell is genetically unstable and hence can mutate faster. They can also modify their microenvironment and escape immune surveillance. The difficulties in treating cancer are further compounded by the emergence of rapid resistance to anticancer drugs. The most common ways to attain resistance in cancer cells include alteration in drug transport and metabolism, modification of drug target, elevated DNA damage response, or...
3.6K
Cell Polarization by Rho Proteins01:21

Cell Polarization by Rho Proteins

Cell polarity is the asymmetric distribution of cellular and membrane components, making one side of the cell different from the other. This polarity is essential to many processes such as embryogenesis, axon migration, glucose transport across epithelial cells, and directional cell migration. A migrating cell responds to intracellular or extracellular signals via molecular cascades that reorganize the actin cytoskeleton to establish this polarity. In these cells, the Rho family proteins Cdc42,...
3.4K
Small GTPases - Ras and Rho01:24

Small GTPases - Ras and Rho

Ras and Rho are small monomeric GTPases that act downstream of receptor tyrosine kinase (RTK) and regulate various cellular processes. These GTPases switch between active and inactive states by binding to guanine nucleotides.
Three regulatory proteins control their activity:
5.1K