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Updated: Jun 18, 2026
09:24
Systemic Injection of Neural Stem/Progenitor Cells in Mice with Chronic EAE
Published on: April 15, 2014
Japanese encephalitis virus induce immuno-competency in neural stem/progenitor cells
Sulagna Das1, Debapriya Ghosh, Anirban Basu
1National Brain Research Centre, Manesar, Haryana, India.
Plos One
|December 4, 2009
Summary
Neural stem/progenitor cells (NSPCs) become immunogenic when infected with Japanese encephalitis virus (JEV). JEV-infected NSPCs stimulate T cell proliferation, suggesting a new role in the central nervous system immune response.
Area of Science:
- Neuroscience
- Immunology
- Stem Cell Biology
Background:
- Neural stem/progenitor cells (NSPCs) have low immunogenicity and are used in transplantation.
- However, inflammation can induce NSPC immunomodulatory functions and antigen expression.
- NSPCs are targets for viral infections, necessitating study of their immune response.
Purpose of the Study:
- To investigate the immunological properties of NSPCs in response to viral infection.
- To understand how Japanese encephalitis virus (JEV) affects NSPC immunogenicity.
Main Methods:
- Used in vivo mouse models and in vitro neurosphere models of JEV infection.
- Analyzed MHC-I and costimulatory molecule expression (CD40, CD80, CD86) via flow cytometry and microscopy.
- Measured pro-inflammatory cytokine/chemokine production and T cell proliferation (Mixed Lymphocyte Reaction, CFSE).
Main Results:
- JEV infection increased MHC-I and costimulatory molecule expression on NSPCs.
- Infected NSPCs produced pro-inflammatory cytokines and chemokines.
- NSPCs stimulated allogenic T cell proliferation and IL-2 production upon JEV infection.
Conclusions:
- JEV infection induces immunogenicity in NSPCs.
- Infected NSPCs may act as immunogenic cells, contributing to innate and adaptive immunity.
- NSPCs could function as non-professional antigen-presenting cells in the CNS post-viral infection.
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