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Related Concept Videos

Circadian Rhythms and Gene Regulation02:19

Circadian Rhythms and Gene Regulation

The biological clock is involved in many aspects of regulating complex physiology in all animals. It was in 1935 when German zoologists, Hans Kalmus and Erwin Bünning, discovered the existence of circadian rhythm in Drosophila melanogaster. However, the internal molecular mechanisms behind the circadian clock remained a mystery until 1984, when Jeffrey C. Hall, Michael Rosbash, and Michael W. Young discovered the expression of the Per gene oscillating over a 24-hour cycle. In subsequent years,...
Circadian Rhythms and Gene Regulation02:19

Circadian Rhythms and Gene Regulation

The biological clock is involved in many aspects of regulating complex physiology in all animals. It was in 1935 when German zoologists, Hans Kalmus and Erwin Bünning, discovered the existence of circadian rhythm in Drosophila melanogaster. However, the internal molecular mechanisms behind the circadian clock remained a mystery until 1984, when Jeffrey C. Hall, Michael Rosbash, and Michael W. Young discovered the expression of the Per gene oscillating over a 24-hour cycle. In subsequent years,...
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The circadian—or biological—clock is an intrinsic, timekeeping, molecular mechanism that allows plants to coordinate physiological activities over 24-hour cycles called circadian rhythms. Photoperiodism is a collective term for the biological responses of plants to variations in the relative lengths of dark and light periods. The period of light-exposure is called the photoperiod.

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Single-cell Resolution Fluorescence Live Imaging of Drosophila Circadian Clocks in Larval Brain Culture
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Published on: January 19, 2018

Quantification of circadian rhythms in single cells.

Pål O Westermark1, David K Welsh, Hitoshi Okamura

  • 1Institute for Theoretical Biology, Humboldt University, Berlin, Germany. p.westermark@biologie.hu-berlin.de

Plos Computational Biology
|December 4, 2009
PubMed
Summary

This study used bioluminescence to analyze circadian clock gene expression in mouse cells. Mathematical models characterized cell behavior, but could not definitively determine if the circadian clock is damped or self-sustained.

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Last Updated: Jun 18, 2026

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Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures
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Monitoring Cell-autonomous Circadian Clock Rhythms of Gene Expression Using Luciferase Bioluminescence Reporters
10:38

Monitoring Cell-autonomous Circadian Clock Rhythms of Gene Expression Using Luciferase Bioluminescence Reporters

Published on: September 27, 2012

Area of Science:

  • Chronobiology
  • Molecular Biology
  • Systems Biology

Background:

  • Bioluminescence imaging enables precise monitoring of circadian rhythms in individual cells.
  • The suprachiasmatic nucleus (SCN) and fibroblasts are key models for studying circadian clock mechanisms.

Purpose of the Study:

  • To quantitatively characterize circadian clock parameters in mouse SCN neurons and fibroblasts using bioluminescence data.
  • To differentiate between damped and self-sustained oscillator models for the single-cell circadian clock.

Main Methods:

  • Analysis of bioluminescence data from Per gene expression in mouse SCN neurons and fibroblasts.
  • Application of two mathematical models: a damped oscillator driven by noise and a self-sustained noisy oscillator.
  • Quantitative characterization of wild-type and mutant cell behaviors.

Main Results:

  • Both mathematical models effectively described the experimental bioluminescence data.
  • The models allowed for quantitative comparison of wild-type and mutant cellular circadian parameters.
  • Current data are insufficient to definitively classify the single-cell circadian clock as damped or self-sustained.

Conclusions:

  • The study highlights the need for further experiments to resolve the damped vs. self-sustained nature of the single-cell circadian clock.
  • Testing model predictions under periodic entrainment (zeitgeber) is proposed as a method to distinguish between oscillator types.
  • Accurate characterization of circadian clock dynamics in single cells remains an active area of research.