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Resveratrol down-regulates survivin and induces apoptosis in human multidrug-resistant SPC-A-1/CDDP cells

Weiguo Zhao1, Pengtao Bao, Haowen Qi

  • 1Department of Respiratory Medicine, The Second Affiliated Hospital of Chinese PLA General Hospital, Beijing, P.R. China. zhaowg2008@qq.com

Oncology Reports
|December 4, 2009
PubMed

Insights

Resveratrol effectively combats multidrug-resistant lung cancer by inhibiting cell growth and inducing apoptosis. This natural compound also enhances chemotherapy efficacy and reduces tumor size in preclinical models.

Area of Science:

  • Oncology
  • Pharmacology
  • Molecular Biology

Background:

  • Multidrug resistance (MDR) in non-small cell lung cancer (NSCLC) poses a significant therapeutic challenge.
  • Resveratrol, a natural polyphenol, has demonstrated potential anti-cancer properties.
  • Investigating resveratrol's efficacy against MDR NSCLC is crucial for developing novel treatment strategies.

Purpose of the Study:

  • To evaluate the in vitro and in vivo effects of resveratrol on multidrug-resistant human non-small cell lung cancer (NSCLC) cells (SPC-A-1/CDDP).
  • To determine resveratrol's impact on cell proliferation, apoptosis, cell cycle, chemosensitivity, and tumor growth.
  • To elucidate the role of survivin expression in resveratrol-mediated anti-cancer effects.

Main Methods:

  • In vitro treatment of SPC-A-1/CDDP cells with varying concentrations of resveratrol.
  • In vivo studies using nude mice xenograft models implanted with SPC-A-1/CDDP cells, treated with resveratrol orally.
  • Assessment of cell proliferation, apoptosis, cell cycle distribution, drug IC50 values, tumor volume, and survivin expression.

Main Results:

  • Resveratrol significantly inhibited SPC-A-1/CDDP cell proliferation and induced apoptosis in a dose- and time-dependent manner.
  • Resveratrol treatment led to cell cycle arrest at G0-G1 and G2/M phases.
  • Resveratrol decreased the IC50 values of cisplatin, gefitinib, and paclitaxel, indicating enhanced chemosensitivity, and reduced tumor volume in vivo.
  • Resveratrol downregulated survivin expression in a dose-dependent manner, correlating with apoptosis induction.

Conclusions:

  • Resveratrol exhibits significant anti-proliferative and pro-apoptotic effects against multidrug-resistant human NSCLC cells.
  • Resveratrol enhances the efficacy of conventional chemotherapeutic agents and possesses anti-tumor activity in vivo.
  • Downregulation of survivin expression is a key mechanism by which resveratrol induces apoptosis in MDR NSCLC.

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