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Related Experiment Video

Updated: Jun 18, 2026

A Simple High Efficiency Protocol for Pancreatic Islet Isolation from Mice
06:56

A Simple High Efficiency Protocol for Pancreatic Islet Isolation from Mice

Published on: August 30, 2019

A practical guide to rodent islet isolation and assessment.

Jeffrey D Carter, Stacey B Dula, Kathryn L Corbin

    Biological Procedures Online
    |December 4, 2009
    PubMed
    Summary

    This review details methods for isolating functional pancreatic islets for diabetes research. It covers collagenase digestion, density gradient purification, and viability assessments for rodent models.

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    Area of Science:

    • Endocrinology and Metabolism
    • Cell Biology
    • Diabetes Research

    Background:

    • Pancreatic islets of Langerhans are crucial for blood glucose regulation and are central to diabetes research.
    • Islet isolation for study requires intricate purification of viable and functional cells.
    • Rodent models are frequently used to study islet function and diabetes.

    Purpose of the Study:

    • To provide a comprehensive review of mouse and rat islet isolation techniques.
    • To detail methods for assessing islet viability and function.
    • To offer a practical protocol for obtaining high-quality islets for research.

    Main Methods:

    • Collagenase digestion of pancreatic tissue.
    • Density gradient centrifugation for islet purification.

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    Pancreatic Islet Isolation and Purification from Lewis Rats Using Enzymatic Digestion and Density-Gradient Separation
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    Pancreatic Islet Isolation and Purification from Lewis Rats Using Enzymatic Digestion and Density-Gradient Separation

    Published on: April 3, 2026

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    Last Updated: Jun 18, 2026

    A Simple High Efficiency Protocol for Pancreatic Islet Isolation from Mice
    06:56

    A Simple High Efficiency Protocol for Pancreatic Islet Isolation from Mice

    Published on: August 30, 2019

    Pancreatic Islet Isolation and Purification from Lewis Rats Using Enzymatic Digestion and Density-Gradient Separation
    07:44

    Pancreatic Islet Isolation and Purification from Lewis Rats Using Enzymatic Digestion and Density-Gradient Separation

    Published on: April 3, 2026

  • Assessment of islet viability using visual inspection and fluorescent markers.
  • Measurement of glucose-stimulated insulin secretion and intracellular calcium.

    • Main Results:

    • The review outlines critical factors in islet isolation, including collagenase selection and digestion parameters.
    • It discusses various purification strategies, emphasizing density gradient methods.
    • Established techniques for evaluating islet viability and function are presented.

    Conclusions:

    • Successful rodent islet isolation is achievable with careful attention to protocol details.
    • High-quality islets are essential for accurate in vitro and in vivo research.
    • This review serves as a foundational resource for researchers in the field of diabetes.